Drug-Loaded Elastin-Like Polypeptide-Collagen Hydrogels with High Modulus for Bone Tissue Engineering

Macromolecular Bioscience
Pallabi PalAmol V Janorkar

Abstract

Emphasizing the role of hydrogel stiffness and cellular differentiation, this study develops collagen and elastin-like polypeptide (ELP)-based bone regenerative hydrogels loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) and doxycycline with mechanical properties suitable for osteogenesis. The drug-incorporated collagen-ELP hydrogels has significantly higher modulus of 35 ± 5 kPa compared to collagen-only hydrogels. Doxycycline shows a bi-phasic release with an initial burst release followed by a gradual release, while rhBMP-2 exhibits a nearly linear release profile for all hydrogels. The released doxycycline shows anti-microbial activity against Pseudomonas aeruginosa, Streptococcus sanguinis, and Escherichia coli. Microscopic observation of the hydrogels reveals their interconnected, macroporous, 3D open architecture with pore diameters between 160 and 400 µm. This architecture supports human adipose-derived stem cell attachment and proliferation from initial days of cell seeding, forming a thick cellular sheath by day 21. Interestingly, in collagen and collagen-ELP hydrogels, cell morphology is elongated with stretched slender lamellipodial formation, while cells assemble as spheroidal aggregates in cross...Continue Reading

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Citations

Jan 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Falko DoberenzThomas Groth
Dec 6, 2019·Tissue Engineering. Part C, Methods·Tim KlüterSabine Fuchs
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May 8, 2021·Biomacromolecules·Tianqi NieZhiyu He
Nov 20, 2021·ACS Biomaterials Science & Engineering·Marjan BahraminasabMohammad Sadegh Nourbakhsh

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