Drug-plasma protein binding assay by electrokinetic chromatography-frontal analysis

Electrophoresis
Yasushi IshihamaNaoki Asakawa

Abstract

We developed a rapid, microscale and reliable analytical method for binding of drugs to plasma proteins using capillary electrophoresis (CE) with ionic cyclodextrins (CD) combined with frontal analysis. These CDs were used as pseudostationary phases of electrokinetic chromatography (EKC). The CD-modified EKC (CDEKC) approach allowed us to separate anionic drugs from plasma proteins, whereas CZE could not separate these drugs from plasma proteins because they had a similar mobility like plasma proteins. CDs uniquely interact with these drugs but not with plasma proteins. Therefore, CDEKC could be coupled with frontal analysis to measure the binding of anionic drugs to plasma proteins. The binding values obtained by CDEKC were highly consistent with those determined by the ultrafiltration method. Our CDEKC approach should expand the applicability of CE to protein binding analysis.

References

Sep 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·R J TaitJ F Stobaugh
Sep 11, 1992·Journal of Chromatography·J C KraakH Poppe
Oct 1, 1981·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·K Uekama
Jun 13, 1998·Analytical Chemistry·H KatayamaN Asakawa

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Citations

Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C BertucciV Andrisano
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shufeng Zhou
Jun 24, 2006·Journal of Pharmaceutical Sciences·Florin Marcel MusteataGerald T Miwa
Sep 1, 2006·Electrophoresis·María A Martínez-GómezMaria J Medina-Hernández
May 30, 2006·Electrophoresis·Jesper Ostergaard, Niels H H Heegaard
Jun 15, 2004·Journal of Pharmaceutical and Biomedical Analysis·Dawei Zhou, Famei Li
Jul 1, 2005·Drug Discovery Today. Technologies·Hong Wan, Richard A Thompson
Sep 25, 2020·Bioelectrochemistry·Alexander M FirsovYuri N Antonenko

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