Drug resistance and membrane alteration in mutants of mammalian cells

Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie
V Ling

Abstract

Independent colchicine-resistant (CHR) mutants of Chinese hamster ovary cells displaying reduced permeability to colchicine have been isolated. A distinguishing feature of these membrane-altered mutants is their pleiotropic cross-resistance to a variety of unrelated compounds. Genetic characterization of the CHR lines indicate that colchicine resistance and cross-resistance to other drugs are of a dominant nature in somatic cell hybrids. Revertants of CHR have been isolated which display decreased resistance to colchicine and a corresponding decrease in resistance to other drugs. These results strongly suggest that colchicine resistance and the pleiotropic cross-resistance are the result of the same mutation(s). Biochemical studies indicate that although colchicine is transported into our cells by passive diffusion, no major alterations in the membrane lipids could be detected in mutant cells. However, there appears to be an energy-dependent process in these cells which actively maintains a permeability barrier against colchicine and other drugs. The CHR cells might be altered in this process. A new glycoprotein has been identified in mutant cell membranes which is not present in parental cells, and is greatly reduced in revert...Continue Reading

References

Feb 18, 1972·Science·S J Singer, G L Nicolson
May 1, 1972·Chemistry and Physics of Lipids·B G McFarland
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C G Gahmberg, S I Hakomori
Oct 1, 1974·Differentiation; Research in Biological Diversity·Y LangelierC Brailovsky
Feb 12, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·T M AndrewsR W Watts
Mar 30, 1974·Experimental Cell Research·J R Warr, D Gibbons
Jul 12, 1974·Biochimica Et Biophysica Acta·M Grunze, B Deuticke
Feb 1, 1974·Journal of Cellular Physiology·V Ling, L H Thompson

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Citations

Jan 1, 1992·Cancer Chemotherapy and Pharmacology·C P VendrikP A Steerenberg
Jan 1, 1984·Breast Cancer Research and Treatment·V LingN Kartner
Jan 1, 1979·The International Journal of Biochemistry·H Ceri, J A Wright
Dec 1, 1992·Pharmacology & Therapeutics·K D TewL A Speicher
Jan 1, 1985·Pharmacology & Therapeutics·J R Riordan, V Ling
Jun 13, 1998·The Journal of Experimental Medicine·N AltanS M Simon
May 1, 1977·Journal of Cellular Physiology·V Ling
May 1, 1986·International Journal of Radiation Oncology, Biology, Physics·K Wallner, G C Li
Sep 1, 1991·Journal of Pediatric Surgery·M P LaQuagliaJ L Biedler
Dec 1, 1979·Genetical Research·S Brabbs, J R Warr
Dec 1, 1978·Journal of Cellular Physiology·J L Mandel, W F Flintoff
Nov 15, 1987·International Journal of Cancer. Journal International Du Cancer·H DiddensD Niethammer
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·I PályiM Bak
Jan 1, 1978·Journal of Supramolecular Structure·R H Peterson, J L Biedler
Jul 25, 2006·Pflügers Archiv : European journal of physiology·Jonathan A Sheps, Victor Ling
Mar 1, 1978·Somatic Cell Genetics·V Ling, R M Baker
Sep 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu S Massino, B P Kopnin
Jun 29, 2001·The Journal of Pharmacy and Pharmacology·Z LiuX Y Wu
Jun 24, 2008·Critical Reviews in Toxicology·Edward J Calabrese
Nov 13, 2020·FEBS Letters·Thomas StocknerLutz Schmitt
Jul 9, 2009·Chemical Reviews·Paul D W Eckford, Frances J Sharom
May 11, 1999·Drug Discovery Today·S M Simon
May 1, 1985·Biochemical Pharmacology·R A WillsonU Muller-Eberhard
Feb 18, 1988·Biochimica Et Biophysica Acta·A L ArsenaultN Kartner
Nov 11, 1976·Biochimica Et Biophysica Acta·R L Juliano, V Ling

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