Drug resistance marker-aided genome shuffling to improve acetic acid tolerance in Saccharomyces cerevisiae

Journal of Industrial Microbiology & Biotechnology
Dao-Qiong ZhengYu-Hua Zhao

Abstract

Acetic acid existing in a culture medium is one of the most limiting constraints in yeast growth and viability during ethanol fermentation. To improve acetic acid tolerance in Saccharomyces cerevisiae strains, a drug resistance marker-aided genome shuffling approach with higher screen efficiency of shuffled mutants was developed in this work. Through two rounds of genome shuffling of ultraviolet mutants derived from the original strain 308, we obtained a shuffled strain YZ2, which shows significantly faster growth and higher cell viability under acetic acid stress. Ethanol production of YZ2 (within 60 h) was 21.6% higher than that of 308 when 0.5% (v/v) acetic acid was added to fermentation medium. Membrane integrity, higher in vivo activity of the H+-ATPase, and lower oxidative damage after acetic acid treatment are the possible reasons for the acetic acid-tolerance phenotype of YZ2. These results indicated that this novel genome shuffling approach is powerful to rapidly improve the complex traits of industrial yeast strains.

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Citations

Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Oct 31, 2014·Journal of Industrial Microbiology & Biotechnology·Hiroyuki InoueShigeki Sawayama
Aug 6, 2014·Journal of Biotechnology·Xiangyong LiuZhaojie Zhang
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
Apr 26, 2014·Environmental Microbiology·Jieun AnWonja Choi
Apr 15, 2014·FEMS Microbiology Reviews·Jan SteenselsKevin J Verstrepen
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Jan 13, 2018·Frontiers in Bioengineering and Biotechnology·Kentaro InokumaAkihiko Kondo
Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
May 16, 2014·Applied Biochemistry and Biotechnology·Qingling WangXinle Liang
Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Jun 8, 2018·Journal of Industrial Microbiology & Biotechnology·Victor E Balderas-HernándezRadhakrishnan Mahadevan
Jan 8, 2019·Journal of Biotechnology·Eun Joong OhYong-Su Jin

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