PMID: 9439766May 1, 1997Paper

Drug specific antibodies: T-cell epitope-lipopeptide conjugates are potent adjuvants for small antigens in vivo and in vitro

International Journal of Immunopharmacology
K MittenbühlerP Hoffmann

Abstract

To generate conventional or monoclonal antibodies for the serological detection of drugs, antibiotics, toxins and other low molecular mass substances, a suitable and effective adjuvant is needed. Lipopeptides derived from a major component of the bacterial cell wall constitute potent nontoxic and nonpyrogenic immunoadjuvants when mixed with conventional antigens. Here we demonstrate that the synthetic lipopeptide N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2R,S)-propyl]-(R)-cysteinyl- serine (P3CS) coupled to a Th-cell epitope (P3CS-Th) can efficiently enhance the specific immune response against low molecular weight compounds in different species. In the presence of the synthetic lipopeptide P3CS-Th, the peptides which are per se non-immunogenic stimulated a specific humoral immune response in mice after intraperitoneal application. Mixtures containing adjuvants without the Th sequence showed no significant antibody induction. A marked enhancement of the humoral immune response was obtained with the low molecular mass antigens Iturin AL, Herbicolin A and Microcystin (MLR) coupled to poly-l-lysin (MLR-PLL), in rabbits and in chickens. Lipopeptide-Th cell epitope conjugates also constituted adjuvants for the in vitro immunization of e...Continue Reading

References

Jun 1, 1992·Research in Immunology·W G Bessler, G Jung
Mar 1, 1990·FEMS Microbiology Letters·C MartinJ Weckesser
Sep 13, 1996·Journal of Immunological Methods·P HoffmannW G Bessler

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Citations

Mar 24, 2004·Trends in Biotechnology·Pooja Singh, Swaranjit Singh Cameotra
Mar 10, 2000·International Journal of Immunopharmacology·W BaierK Mittenbühler
Sep 28, 2002·International Immunopharmacology·M R MüllerK Mittenbühler
Jun 5, 2015·World Journal of Microbiology & Biotechnology·Seema PatelJ Satya Eswari
Jul 2, 2003·FEMS Immunology and Medical Microbiology·K MittenbühlerM Huber
Jun 24, 2018·Scandinavian Journal of Immunology·Naoto YoshinoShigehiro Sato
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
Feb 18, 2003·Chemical Research in Toxicology·J S Metcalf, G A Codd

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