Drug specific cytotoxic T-cells in the skin lesions of a patient with toxic epidermal necrolysis

The Journal of Investigative Dermatology
Amal NassifJean-Claude Roujeau

Abstract

Toxic epidermal necrolysis is an extremely severe drug reaction, manifesting itself by widespread apoptosis of keratinocytes, generally considered to result from Fas/CD95-FasLigand interaction, but of unknown primary mechanism. We looked at the role of cells present in the skin blisters as probable effectors of this immune reaction. In a patient suffering from cotrimoxazole-induced toxic epidermal necrolysis, blister fluid cells were phenotyped by FACS and tested without prior restimulation for cytotoxicity on autologous and allogeneic cells in the presence of the drug. Blister fluid lymphocytes were predominantly CD8+, DR+, CLA+, CD56+ T lymphocytes, perforin positive and expressing preferentially two Vbeta chains of the T cell receptor repertoire. These lymphocytes were cytotoxic only in the presence of the drug towards autologous EBV transformed lymphocytes and towards allogeneic cells sharing HLA-Cw4. Cytotoxicity occurred in the presence of either cotrimoxazole, sulfamethoxazole, or the nitroso metabolite of sulfamethoxazole, but not with the hydroxylamine metabolite of sulfamethoxazole. The lysis was blocked by an anti-MHC class I monoclonal antibody. It was abolished by EGTA and CMA, but neither by anti-fas, brefeldin A,...Continue Reading

References

Jan 1, 1979·The British Journal of Dermatology·A Lyell
Dec 14, 1995·The New England Journal of Medicine·J C RoujeauF Locati
Nov 10, 1994·The New England Journal of Medicine·J C Roujeau, R S Stern
Apr 1, 1996·The British Journal of Dermatology·C PaulJ C Roujeau
Jul 1, 1997·The Journal of Clinical Investigation·B SchnyderW J Pichler
May 21, 1998·Lancet·D S Becker
Nov 21, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B SchnyderW J Pichler
Jan 12, 2000·The Journal of Allergy and Clinical Immunology·L LeyvaC Juárez
Nov 15, 2000·Lancet·S R KnowlesN H Shear

❮ Previous
Next ❯

Citations

Sep 25, 2002·Allergy·W PichlerA Helbling
Feb 23, 2007·The British Journal of Ophthalmology·Mayumi UetaShigeru Kinoshita
Aug 12, 2006·Dermatology : International Journal for Clinical and Investigative Dermatology·Yuichi TerakiTetsuo Shiohara
Apr 6, 2006·The AAPS Journal·Sanjoy Roychowdhury, Craig K Svensson
Apr 6, 2006·The AAPS Journal·Joseph P SandersonB Kevin Park
Nov 2, 2011·Drug Metabolism and Pharmacokinetics·Chun-Yu WeiYuan-Tsong Chen
Jul 7, 2010·Pharmacogenomics·Elizabeth J Phillips, Simon A Mallal
Sep 17, 2013·Cell Biochemistry and Biophysics·I A AimolaN B Afolabi-Balogun
Oct 25, 2011·Annual Review of Pharmacology and Toxicology·Mandvi BharadwajJames McCluskey
Mar 21, 2007·Expert Opinion on Drug Safety·Dean J NaisbittB Kevin Park
Jul 27, 2006·Expert Opinion on Drug Metabolism & Toxicology·Farah D KhanCraig K Svensson
Jun 13, 2015·The Journal of Investigative Dermatology. Symposium Proceedings·Fangping YangQinghe Xing
Jan 16, 2016·The British Journal of Dermatology·Y K HengJ-C Roujeau
Nov 13, 2012·Current Opinion in Immunology·Patricia T IllingJames McCluskey
May 1, 2012·Journal of Dermatological Science·Wen-Hung Chung, Shuen-Iu Hung
Apr 18, 2008·The Ocular Surface·Darren G Gregory
Nov 18, 2011·Journal of the American Academy of Dermatology·Laurence Valeyrie-AllanorePierre Wolkenstein
Jan 7, 2016·Chemical Research in Toxicology·Richard A ThompsonDominic P Williams
Apr 23, 2003·The American Journal of Pathology·Riichiro AbeHiroshi Shimizu
Sep 18, 2010·Chemico-biological Interactions·B K ParkD P Williams
Jul 9, 2010·The Medical Clinics of North America·Werner J PichlerDaniel Yerly
Feb 18, 2010·The Journal of Allergy and Clinical Immunology·J Luis CastrejonDean J Naisbitt
Feb 6, 2010·The Journal of Allergy and Clinical Immunology·Esther MorelTeresa Bellón
Sep 22, 2009·Burns : Journal of the International Society for Burn Injuries·Mario LissiaCorrado Rubino
Jul 1, 2009·Immunology and Allergy Clinics of North America·Priska LochmatterWerner J Pichler
Oct 16, 2004·The Journal of Investigative Dermatology·Amal NassifJean-Claude Roujeau
Mar 17, 2007·The Journal of Investigative Dermatology·Junko Murata, Riichiro Abe
Aug 19, 2007·The Journal of Allergy and Clinical Immunology·Chih-Wen Ou YangYuan-Tsong Chen
Jul 3, 2007·Journal of Dermatological Science·Daisuke NishioYoshiki Tokura
Mar 9, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Khalid A Al-JohaniStephen R Porter
Mar 10, 2016·Seminars in Ophthalmology·Hajirah N Saeed, James Chodosh
Jan 17, 2007·Journal of the American Academy of Dermatology·Frederick A PereiraDavid M Rosmarin
Aug 27, 2005·Oral Diseases·P FarthingC Scully
Aug 10, 2005·The British Journal of Dermatology·T A ChaveP E Hutchinson
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P S FriedmannR St C Barnetson
Dec 2, 2014·Journal of the American Academy of Dermatology·Hisato IrikiKeisuke Nagao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
Erik LetkoC Stephen Foster
© 2022 Meta ULC. All rights reserved