DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer

Cancer Cell International
Shuhang QinShuzhong Yao

Abstract

The pathogenesis and developmental mechanism of early-stage (FIGO 2009 IA2-IIA2) cervical cancer (CC) remain unclear. Seeking novel molecular biomarkers based on The Cancer Genome Atlas (TCGA) will facilitate the understanding of CC pathogenesis and help evaluate early-stage CC prognosis. To identify prognosis-related genes in early-stage CC, we analyzed TCGA mRNA-seq data and clinical data by univariate Cox and Kaplan-Meier plotter analyses. Differential expression analysis identified upregulated genes in early-stage CC. Combined with the genes correlated with unfavorable prognosis, we selected desmoglein-2 (DSG2) for further investigation. To detect DSG2 expression in early-stage CC, we used immunohistochemistry (IHC), quantitative real-time PCR (qRT-PCR) and western blotting. The relationship between the expression of DSG2 and clinical features was analyzed by the Chi square test. Cox analysis was applied to assess the relationship between CC overall survival (OS) and risk factors. The correlations between DSG2 expression and CC cell line proliferation and migration were investigated with Cell Counting Kit-8 (CCK-8) and migration assays. There were 416 prognosis-related genes in early-stage CC. DSG2, matrix metallopeptidase ...Continue Reading

References

Jan 28, 2004·Journal of Cutaneous Pathology·Hjalmar KurzenWolfgang Hartschuh
Aug 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Masakazu YashiroKosei Hirakawa
Mar 16, 2007·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Dec 19, 2008·BMC Cancer·Vishnu C RamaniRandy S Haun
May 22, 2009·Cell Adhesion & Migration·Donna Brennan, Mÿ G Mahoney
Dec 4, 2014·Pathology, Research and Practice·Friederike SaaberIver Petersen
Oct 23, 2016·Oncotarget·Andrew M OvermillerMỹ G Mahoney
Jan 24, 2017·Nature·UNKNOWN Cancer Genome Atlas Research NetworkUNKNOWN Washington University in St Louis
Mar 10, 2017·Molecular Carcinogenesis·Katharina HützVolker Spindler
Sep 2, 2017·OncoTargets and Therapy·Guangxin ZhouTiantian Cui
Aug 22, 2018·Current Medical Science·Bang-Xing Huang, Fang Fang

❮ Previous
Next ❯

Methods Mentioned

BETA
hysterectomy
PCR
electrophoresis
transfection

Software Mentioned

cBioPortal
SPSS
R package “ DEseq
R
Metascape

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.