DSGOST regulates resistance via activation of autophagy in gastric cancer

Cell Death & Disease
Tae Woo KimSeong-Gyu Ko

Abstract

Danggui-Sayuk-Ga-Osuyu-Saenggang-Tang (DSGOST in Korean, Danggui-Sini-Jia-Wuzhuyu-Shengian-Tang in Chinese, and Tokishigyakukagoshuyushokyoto (TJ-38) in Japanese), a well-known traditional Korean/Chinese/Japanese medicine, has long been used to treat vascular diseases such as Raynaud's phenomenon (RP). However, anticancer effect of DSGOST remains elusive. In this study, we checked if DSGOST has an anticancer effect against gastric cancer cells, and investigated the mechanisms underlying DSGOST resistance. Moreover, DSGOST regulates chemoresistance in cisplatin-treated gastric cancer cells. Interestingly, DSGOST treatment induced the accumulation of GFP-LC3 puncta and increased the level of autophagy markers, such as LC3-II, ATG5, and Beclin-1, indicating activated autophagy. Furthermore, DSGOST could activate epithelial-to-mesenchymal transition (EMT) and exosomes via induction of autophagy. DSGOST in combination with TGFβ also induced autophagy and EMT. However, autophagy inhibition induces DSGOST-mediated cell death in gastric cancer cells. In addition, autophagy inhibition blocks the activation of DSGOST-mediated EMT markers including N-cadherin, Snail, Slug, vimentin, β-catenin, p-Smad2, and p-Smad3. Taken together, these f...Continue Reading

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Citations

May 21, 2020·Journal of Experimental & Clinical Cancer Research : CR·Yifeng CuiLianxin Liu
Mar 1, 2019·Molecular Medicine Reports·Meiyan LiuLi Cai
Sep 2, 2020·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Zhong YangMingxu Da
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Jing-Li XuJiang-Jiang Qin
Feb 19, 2021·Journal of Receptor and Signal Transduction Research·Lijuan Ma, Youhui Wang
May 13, 2021·International Journal of General Medicine·Ting XiuFan-Bo Jing
Oct 1, 2021·Recent Patents on Anti-cancer Drug Discovery·Sheng-Xiong ZhangLi-Zhu Lin

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Methods Mentioned

BETA
lipidation
Fluorescence microscopy
transfection
co-immunoprecipitation
immunoprecipitation
ELISA
PCR
confocal microscopy
pull down

Software Mentioned

DSGOST

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