DSPP-MMP20 gene silencing downregulates cancer stem cell markers in human oral cancer cells

Cellular & Molecular Biology Letters
Nikolaos G NikitakisKalu U E Ogbureke

Abstract

Recent findings indicate that dentin sialophosphoprotein (DSPP) and matrix metalloproteinase (MMP) 20 interact in oral squamous cell carcinoma (OSCC). The objective of this study was to determine the effects of DSPP/MMP20 gene silencing on oral cancer stem cell (OCSC) markers. The expression of well-established OCSC markers: ABCG2; ALDH1; CD133; CD44; BMI1; LGR4, and Podoplanin in DSPP/MMP20-silenced OSCC cell line, OSC2, and controls were assayed by western blot (WB), and flow cytometry techniques. The sensitivity of OSC2 cells to cisplatin following DSPP/MMP20 silencing was also determined. DSPP/MMP20 silencing resulted in downregulation of OCSC markers, more profoundly ABCG2 (84%) and CD44 (81%), following double silencing. Furthermore, while treatment of parent (pre-silenced) OSC2 cells with cisplatin resulted in upregulation of OCSC markers, DSPP/MMP20-silenced OSC2 cells similarly treated resulted in profound downregulation of OCSC markers (72 to 94% at 50 μM of cisplatin), and a marked reduction in the proportion of ABCG2 and ALDH1 positive cells (~ 1%). We conclude that the downregulation of OCSC markers may signal a reduction in OCSC population following MMP20/DSPP silencing in OSCC cells, while also increasing their s...Continue Reading

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Citations

Dec 24, 2019·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Mar 2, 2021·Neuroscience Letters·Huasong GaoYi Zhang
Jul 27, 2021·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anamarija HabičBarbara Breznik

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
Protein Assay
FACS
GTPases
xenograft

Software Mentioned

Image J
BD Cell Quest Pro
statistical Packages for the Social Sciences ( SPSS

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