DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton

Nature Microbiology
Andrew R J CursonJonathan D Todd

Abstract

Dimethylsulfoniopropionate (DMSP) is a globally important organosulfur molecule and the major precursor for dimethyl sulfide. These compounds are important info-chemicals, key nutrients for marine microorganisms, and are involved in global sulfur cycling, atmospheric chemistry and cloud formation1-3. DMSP production was thought to be confined to eukaryotes, but heterotrophic bacteria can also produce DMSP through the pathway used by most phytoplankton 4 , and the DsyB enzyme catalysing the key step of this pathway in bacteria was recently identified 5 . However, eukaryotic phytoplankton probably produce most of Earth's DMSP, yet no DMSP biosynthesis genes have been identified in any such organisms. Here we identify functional dsyB homologues, termed DSYB, in many phytoplankton and corals. DSYB is a methylthiohydroxybutryate methyltransferase enzyme localized in the chloroplasts and mitochondria of the haptophyte Prymnesium parvum, and stable isotope tracking experiments support these organelles as sites of DMSP synthesis. DSYB transcription levels increased with DMSP concentrations in different phytoplankton and were indicative of intracellular DMSP. Identification of the eukaryotic DSYB sequences, along with bacterial dsyB, pr...Continue Reading

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Citations

Jan 5, 2019·Angewandte Chemie·Cangsong Liao, Florian P Seebeck
Mar 20, 2018·Nature Reviews. Microbiology·Ursula Hofer
Jun 25, 2019·Science China. Life Sciences·Xiao-Hua ZhangJonathan D Todd
Sep 22, 2019·Nature Microbiology·Beth T Williams, Jonathan D Todd
Sep 6, 2019·Nature Reviews. Microbiology·Mary Ann Moran, Bryndan P Durham
Jan 10, 2019·Frontiers in Microbiology·Jingli LiuXiao-Hua Zhang
Jun 23, 2019·The ISME Journal·Marine LandaMary Ann Moran
Aug 21, 2019·Nature Microbiology·Beth T WilliamsJonathan D Todd
Jan 9, 2021·Environmental Microbiology·Erin L McParlandNaomi M Levine
Sep 18, 2018·Current Biology : CB·Olivier De ClerckJohn H Bothwell
Sep 11, 2020·Marine Environmental Research·Amandine M N CaruanaZouher Amzil
Apr 1, 2021·Environmental Science & Technology·Emanuel MüllerLenny H E Winkel
Apr 4, 2021·Microorganisms·Ji LiuXiao-Hua Zhang
Jul 5, 2021·Marine Pollution Bulletin·Shi-Hai MaoGui-Peng Yang

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Datasets Mentioned

BETA
PRJNA272345

Methods Mentioned

BETA
electrophoresis
PCR
flow cytometry

Software Mentioned

FastTree
BLASTP
OptimumAntigen
Mass Hunter workstation
MIMS
TREE
R
MAFFT
SignalP
HMM

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