PMID: 8958158Jan 1, 1997Paper

DT-Diaphorase maintains the reduced state of ubiquinones in lipid vesicles thereby promoting their antioxidant function

Free Radical Biology & Medicine
L LandiR E Beyer

Abstract

The activity of purified DT-diaphorase in the reduction of ubiquinone homologues of different side-chain length incorporated in uni- and multilamellar vesicles was determined. The direct relationship between the reduced state of ubiquinones and the inhibition of lipid autoxidation induced by thermolabile azocompounds was also demonstrated. Results demonstrate that DT-diaphorase is able to generate and to maintain the reduced, antioxidant form of ubiquinones in both types of vesicles. Furthermore, the results reported herein show that, in the presence of nicotinamide adenine dinucleotide (NADH) and DT-diaphorase, ubiquinol-containing multilamellar vesicles exposed to a lipophilic azocompound did not undergo lipid peroxidation, whereas in vesicles lacking either NADH or DT-diaphorase, thiobarbituric acid reactive substances (TBARS) formation occurred. It is suggested that DT-diaphorase may be responsible for maintaining the reduced state of ubiquinones in various nonmitochondrial cellular membranes.

References

Jan 1, 1978·Biochemical Pharmacology·Y Masuda, T Murano
Nov 15, 1975·FEBS Letters·P Mitchell
Jun 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R E Beyer
Mar 27, 1992·Biochimica Et Biophysica Acta·J Segura-AguilarC Lind
Dec 21, 1992·FEBS Letters·T TakahashiK Goshima
Apr 1, 1992·Chemistry and Physics of Lipids·L LandiG F Pedulli
Jun 1, 1992·Archives of Biochemistry and Biophysics·F AbergL Ernster
Jan 1, 1991·Free Radical Research Communications·L LandiL Masotti
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B FreiB N Ames
Oct 8, 1987·Biochimica Et Biophysica Acta·A KalénG Dallner
Dec 18, 1984·Biochimica Et Biophysica Acta·M A StidhamJ N Siedow
May 13, 1981·Biochimica Et Biophysica Acta·P B Kingsley, G W Feigenson
Nov 1, 1995·Biochimica Et Biophysica Acta·L LandiM C Galli
Jan 1, 1994·Chemistry and Physics of Lipids·M CipolloneL Landi
Jan 1, 1993·Free Radical Research Communications·D FiorentiniL Landi
Mar 1, 1993·Biochimica Et Biophysica Acta·T ShigemuraN Hamasaki
Apr 9, 1962·Biochimica Et Biophysica Acta·L ERNSTERM LJUNGGREN

❮ Previous
Next ❯

Citations

Mar 5, 2004·Chemistry and Physics of Lipids·Yong CuiKyoung-Chan Park
Mar 7, 2001·Antioxidants & Redox Signaling·J M Villalba, P Navas
Feb 10, 2004·Applied and Environmental Microbiology·D F AckerleyA Matin
Jan 15, 2008·Journal of Clinical Biochemistry and Nutrition·Aikkarach KettawanTadashi Okamoto
Apr 10, 2008·Journal of Clinical Biochemistry and Nutrition·Aikkarach KettawanTadashi Okamoto
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·R T MatthewsM F Beal
Jul 30, 2014·Rejuvenation Research·Dileep Kumar, Syed Ibrahim Rizvi
Nov 24, 2004·Drug Metabolism Reviews·David Ross
Oct 3, 2015·World Neurosurgery·İsmail GülşenMurat Ç Rağbetli
Dec 21, 2010·Experimental Neurology·P A Adlard, A I Bush
May 14, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Reem H Elbekai, Ayman O S El-Kadi
May 8, 2007·Mitochondrion·Plácido NavasRafael de Cabo
Oct 11, 2003·Journal of Biochemistry and Molecular Biology·Wook Hyun KimYe Sun Han
Feb 6, 2004·Biochimica Et Biophysica Acta·Mikael TurunenGustav Dallner
Mar 2, 1999·Archives of Biochemistry and Biophysics·Y S MaE R Stadtman
Jan 10, 2006·Methods in Enzymology·Nandita ShangariPeter J O'Brien
Feb 15, 2013·Toxicology and Industrial Health·P V KiruthigaK Pandima Devi
Jun 15, 2007·Toxicologic Pathology·Lauren M Aleksunes, José E Manautou
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kyeong-Ah Jung, Mi-Kyoung Kwak
Sep 22, 2001·The Journal of Biological Chemistry·R I BelloJ M Villalba
Jul 29, 2010·Critical Reviews in Toxicology·Gary GinsbergBabasaheb Sonawane
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F NavarroJ M Villalba

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.