Dual-cardiac marker capillary waveguide fluoroimmunosensor based on tyramide signal amplification

Analytical and Bioanalytical Chemistry
Aristeidis E NiotisKonstantinos Misiakos

Abstract

The early diagnosis of acute myocardial infarction requires the determination of several markers in serum shortly after its incidence. The markers most widely employed are the isoenzyme MB of creatine kinase (CK-MB) and the cardiac troponin I (cTnI). In the present work, a capillary waveguide fluoroimmunosensor for fast and highly sensitive simultaneous determination of these markers in serum samples is demonstrated. The dual-analyte immunosensor was realized using glass capillaries internally modified with an ultrathin poly(dimethylsiloxane) film by creating discrete bands of analyte-specific antibodies. The capillary was then filled with a mixture of sample and biotinylated detection antibodies followed by reaction with streptavidin-horseradish peroxidase and incubation with a fluorescently labeled tyramide derivative to accumulate fluorescent labels onto immunoreaction bands. Upon scanning the capillary with a laser beam, part of the emitted fluorescence is trapped and waveguided through the capillary wall to a photomultiplier placed on one of its ends. The employment of tyramide signal amplification provided detection limits of 0.2 and 0.5 ng/mL for cTnI and CK-MB, respectively, in a total assay time of 30 min compared to 0...Continue Reading

References

Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Van HeusdenG Smets
Feb 9, 1999·The Journal of Emergency Medicine·D J Robinson, R H Christenson
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E J SpeelP Komminoth
Oct 12, 1999·Analytical Chemistry·T E PlowmanW M Reichert
Feb 19, 2002·Biosensors & Bioelectronics·P S PetrouK Misiakos
Apr 9, 2002·Clinical Biochemistry·Hassan M E Azzazy, Robert H Christenson
Sep 10, 2002·The Journal of Emergency Medicine·John Sarko, Charles V Pollack
Dec 25, 2002·Analytical Chemistry·Christos MastichiadisKonstantinos Misiako
Sep 27, 2003·Analytical Biochemistry·Jingyan WeiGuimin Luo
Jul 9, 2004·Applied and Environmental Microbiology·Adelaide E WarsenDouglas R Call
Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Alan H B WuFred S Apple
Mar 18, 2005·International Journal of Cardiology·Nelly JordanovaKurt Huber
May 16, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Dolci, M Panteghini
Jun 27, 2006·Biosensors & Bioelectronics·Fereidon TorabiMasoud Khayyami
Jan 16, 2007·Analytical Chemistry·Jean-Francois MassonKarl S Booksh
Aug 5, 2008·Molecular and Cellular Probes·Gary J VoraChris Rowe Taitt
Nov 13, 2008·Analytical and Bioanalytical Chemistry·Aristidis E NiotisSotirios E Kakabakos
Jan 17, 2009·Analytical and Bioanalytical Chemistry·Ulrich Friess, Maik Stark

❮ Previous
Next ❯

Citations

Jan 11, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Erina TsutsumiHideaki Hisamoto
Jul 13, 2011·Analytical and Bioanalytical Chemistry·Naoki YanagisawaDebashis Dutta
Aug 24, 2018·Analytical and Bioanalytical Chemistry·Haiying ShenFeng Qu
Aug 15, 2018·3 Biotech·Rajat VashisthaPratyoosh Shukla
Dec 31, 2010·Colloids and Surfaces. B, Biointerfaces·Maria-Elena VlachopoulouSotirios E Kakabakos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.