Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
B M HooksKarel Svoboda

Abstract

Cortical cells integrate synaptic input from multiple sources, but how these different inputs are distributed across individual neurons is largely unknown. Differences in input might account for diverse responses in neighboring neurons during behavior. We present a strategy for comparing the strengths of multiple types of input onto the same neuron. We developed methods for independent dual-channel photostimulation of synaptic inputs using ChR2 together with ReaChR, a red-shifted channelrhodopsin. We used dual-channel photostimulation to probe convergence of sensory information in the mouse primary motor cortex. Input from somatosensory cortex and thalamus converges in individual neurons. Similarly, inputs from distinct somatotopic regions of the somatosensory cortex are integrated at the level of single motor cortex neurons. We next developed a ReaChR transgenic mouse under the control of both Flp- and Cre-recombinases that is an effective tool for circuit mapping. Our approach to dual-channel photostimulation enables quantitative comparison of the strengths of multiple pathways across all length scales of the brain.

Citations

May 7, 2016·Neuroscience and Biobehavioral Reviews·Lieven HuangJames A Bourne
Nov 3, 2016·Frontiers in Cellular Neuroscience·Emmanuelle ChaigneauValentina Emiliani
Apr 20, 2016·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Bryan M Hooks
Feb 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Juhee HaamJerrel L Yakel
Sep 12, 2018·Current Protocols in Neuroscience·Bryan M Hooks
Aug 21, 2020·Physiological Reviews·Jochen F Staiger, Carl C H Petersen
Oct 13, 2017·Frontiers in Neural Circuits·Diana Casas-TorremochaÁngel Núñez
Nov 8, 2017·The Journal of Physiology·Paul G AnastasiadesSimon J B Butt
Jan 26, 2021·The Journal of Physiology·Carmen B SchäferFreek E Hoebeek
Oct 30, 2020·Nature·Seungwon ChoiDavid D Ginty
Mar 20, 2021·PLoS Biology·Min-Hee YiLong-Jun Wu
Jun 23, 2021·Scientific Reports·Clara Rodriguez-SabateManuel Rodriguez
Jul 9, 2021·Frontiers in Systems Neuroscience·Jang Soo YookJinhyun Kim
Jul 23, 2020·Biophysical Journal·Joel C D KaufmannFranz J Bartl

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