Dual-color silver-enhanced in situ hybridization for assessing HER2 gene amplification in breast cancer

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Young Wha KohGyungyub Gong

Abstract

Amplification of the human epidermal growth factor receptor 2 (HER2) gene occurs in 20-25% of breast cancers, and is recognized as a prognostic and predictive marker. HER2 gene amplification, evaluated as a change in protein expression or gene copy number, can be identified by a number of methods. Fluorescence in situ hybridization (FISH) is considered the gold standard for HER2 gene copy number determination; however, a number of impediments prevent its wider use in a clinical setting. The aims of our study were to compare dual-color silver-enhanced in situ hybridization (SISH) with single-color SISH and FISH on formalin-fixed, paraffin-embedded sections, and to validate its use as a routine method for assessing HER2 status in breast cancers. A total of 146 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dual-color SISH. Dual-color SISH and FISH results exhibited a concordance rate of 97% (κ=0.912). A comparison of the single-color SISH method with dual-color SISH showed that 142 of 146 cases were in agreement (97%, κ=0.930). Our results showed that dual-color SISH is a viable alternative to FISH that offers a number of advantages in a clinical setting.

References

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Citations

Apr 23, 2013·BMC Clinical Pathology·Christian OhlschlegelWolfram Jochum
Oct 24, 2013·World Journal of Gastroenterology : WJG·Lucas Faria Abrahão-MachadoCristovam Scapulatempo-Neto
May 4, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Murry W WynesFred R Hirsch
Jan 13, 2016·Virchows Archiv : an International Journal of Pathology·António PolóniaFernando Schmitt
Apr 29, 2014·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Tomoe NakagawaFutoshi Akiyama
Apr 20, 2016·Advanced Drug Delivery Reviews·Dmitriy KhodakovDavid Yu Zhang
Jul 23, 2013·Journal of Clinical Pathology·Cristiana Buzelin NunesHelenice Gobbi
Feb 20, 2015·American Journal of Clinical Pathology·Zaibo LiRohit Bhargava
Jan 13, 2015·Expert Review of Molecular Diagnostics·Francesca Sanguedolce, Pantaleo Bufo
Nov 7, 2019·Journal of Pathology and Translational Medicine·Soomin AhnSo Yeon Park
Aug 30, 2020·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Steffen Grann JensenEstrid Høgdall
May 25, 2016·World Journal of Gastroenterology : WJG·Lucas Faria Abrahao-Machado, Cristovam Scapulatempo-Neto
Apr 7, 2019·Virchows Archiv : an International Journal of Pathology·Mónica CuradoAntónio Polónia
Feb 27, 2020·Archives of Pathology & Laboratory Medicine·Megan TroxellKimberly H Allison

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