Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration

International Journal of Molecular Sciences
Caroline Kohn-PolsterMichael C Hacker

Abstract

Toward the next generation of nerve guidance conduits (NGCs), novel biomaterials and functionalization concepts are required to address clinical demands in peripheral nerve regeneration (PNR). As a biological polymer with bioactive motifs, gelatinous peptides are promising building blocks. In combination with an anhydride-containing oligomer, a dual-component hydrogel system (cGEL) was established. First, hollow cGEL tubes were fabricated by a continuous dosing and templating process. Conduits were characterized concerning their mechanical strength, in vitro and in vivo degradation and biocompatibility. Second, cGEL was reformulated as injectable shear thinning filler for established NGCs, here tyrosine-derived polycarbonate-based braided conduits. Thereby, the formulation contained the small molecule LM11A-31. The biofunctionalized cGEL filler was assessed regarding building block integration, mechanical properties, in vitro cytotoxicity, and growth permissive effects on human adipose tissue-derived stem cells. A positive in vitro evaluation motivated further application of the filler material in a sciatic nerve defect. Compared to the empty conduit and pristine cGEL, the functionalization performed superior, though the autolo...Continue Reading

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Citations

May 2, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Sara GnaviStefania Raimondo
Feb 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hafiz Awais NawazMichael C Hacker
Aug 26, 2021·Biological Chemistry·Jan KrieghoffMichael C Hacker
Oct 1, 2021·Journal of Biomedical Materials Research. Part a·Deanna BousalisChristine E Schmidt

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Methods Mentioned

BETA
scanning electron microscopy
size exclusion chromatography
amidation
rheology
density-gradient centrifugation
ELISA
flow cytometry

Software Mentioned

ImageJ
LabSolutions
BD

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