Dual-crosslinked homogeneous alginate microspheres for mesenchymal stem cell encapsulation

Journal of Materials Science. Materials in Medicine
Jennifer N EtterRachael A Oldinski

Abstract

A smart hydrogel material was used in combination with custom microfluidic devices (MFDs) to create microspheres for human mesenchymal stem cell (MSC) encapsulation. Methods for fabricating homogeneous stimuli-responsive microspheres for MSC encapsulation and cell delivery have gained interest to increase viability and manipulate microencapsulation within microspheres 10-1000 µm in diameter. Herein, MFDs were combined with non-toxic smart hydrogel materials to tune both the size and mechanics of the microspheres. Traditional hydrogels have a single input/stimulus for crosslinking, utilize potentially toxic ultraviolet radiation, and fail to mimic surrounding musculoskeletal tissue mechanics. Thus, it is highly beneficial to encapsulate MSCs inside a mechanically-stable microsphere made from naturally-derived materials. The objectives of this research were to optimize microsphere fabrication techniques using custom microfluidic devices (MFDs), and to encapsulate viable MSCs within visible-light crosslinked smart-alginate microspheres, with tunable mechanical properties. Microsphere production was characterized optically, and MSC viability, post-encapsulation, was verified using a standard florescence assay. Cell viability was ma...Continue Reading

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Citations

Feb 28, 2020·Tissue Engineering. Part a·Stephen HarringtonLisa Stehno-Bittel
Nov 15, 2020·Materials·Miriam Salles PereiraLuiz Anastacio Alves
Aug 28, 2021·International Journal of Molecular Sciences·Teruki Nii, Yoshiki Katayama

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