PMID: 6413657Nov 1, 1983Paper

Dual effects of ascorbate on serotonin and spiperone binding in rat cortical membranes

Journal of Neurochemistry
S F Muakkassah-KellyP Hochstein

Abstract

Ascorbate-induced lipid peroxidation, as measured by malonyldialdehyde (MDA) production, caused irreversible decreases in Bmax of both [3H]5-HT and [3H]spiperone binding. CaCl2 (4 mM) inhibited ascorbate-induced MDA formation at ascorbate concentrations greater than 0.57 mM, but not at less than or equal to 0.57 mM. Under the standard assay conditions (5.7 mM ascorbate and 4 mM CaCl2), CaCl2 inhibited the MDA production caused by ascorbate by 88%, and the loss in [3H]5-HT binding by 57%. Ascorbate still decreased [3H]5-HT binding when lipid peroxidation was completely inhibited by EDTA. This additional effect of ascorbate was reversible after washing the membranes. Other reducing agents (dithiothreitol, glutathione, and metabisulfite) also decreased the binding of [3H]serotonin. In contrast, [3H]spiperone binding was not affected by ascorbate in the absence of lipid peroxidation or by other reducing agents. These experiments demonstrate that ascorbate has a dual and differential effect on serotonin binding sites. First, ascorbate-induced lipid peroxidation irreversibly inactivates both [3H]5-HT and [3H]spiperone binding. Second, independent of lipid peroxidation, there is a direct, reversible effect of ascorbate on [3H]serotoni...Continue Reading

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Citations

Jan 1, 1989·Journal of Neural Transmission. General Section·S C Cheetham, R W Horton
Jan 29, 1986·Brain Research·M R PranzatelliS R Snodgrass
Jan 1, 1986·Neurochemistry International·W WesemannP Hoffmann-Bleihauer
Jan 1, 1987·Neurochemistry International·K von HungenC F Baxter
Jan 1, 1990·Progress in Neurobiology·W Wesemann, N Weiner
Jul 27, 1984·Neuroscience Letters·S Chakrabarti, R Shankar
May 15, 1993·European Journal of Pharmacology·T J WilliamsR J Naylor
Dec 1, 1995·Neurochemistry International·A C Rego, C R Oliveira
Dec 1, 1992·Chemico-biological Interactions·A Van der Vliet, A Bast
Jan 1, 1987·Annals of the New York Academy of Sciences·R E Heikkila, L Manzino
Nov 1, 1985·Pharmacological Research Communications·M SandriniM Baraldi
Jan 1, 1988·Free Radical Research Communications·G R HaenenA Bast
Oct 1, 1986·Journal of Neurochemistry·R W RansomJ C Shih
Jan 6, 1987·Brain Research·R D Todd, R D Ciaranello

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