Dual effects of G-protein activation on Ca-dependent exocytosis in bovine lactotrophs

FEBS Letters
S K SikdarW T Mason

Abstract

The whole-cell patch-clamp technique was used to measure cell membrane capacitance (Cm) to monitor exocytosis in single-cultured bovine prolactin-secreting cells (lactotrophs) of the anterior pituitary. The cells were dialyzed with solutions containing different concentrations of ionised Ca and non-hydrolyzable GTP analogues (GTP-gamma-S and GMP-PNP) to activate G-proteins. We have identified two distinct effects of G-protein activation on Ca-induced exocytosis: (i) the maximum Cm increase due to intracellular Ca-dependent exocytosis was diminished, suggesting an inhibitory role of G-proteins close to the site of granule fusion, while (ii) the rate of Cm increase (delta Cm/delta t) was facilitated, revealing conversely a stimulatory role of G-proteins in the translocation of secretory granules to the fusion sites.

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Citations

Nov 1, 1995·Pflügers Archiv : European journal of physiology·M Rupnik, R Zorec
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·R Zorec
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Oct 1, 1992·The European Journal of Neuroscience·Jean-Marie SontagMarie-France Bader
Jan 1, 1991·Annals of the New York Academy of Sciences·W AlmersF W Tse
Mar 9, 1994·Annals of the New York Academy of Sciences·P M LledoR Zorec
Mar 5, 2016·Journal of Neurochemistry·Peter ThornDamien J Keating
Oct 29, 1990·FEBS Letters·S K SikdarW T Mason
Apr 20, 2004·Glia·Marko KreftRobert Zorec
Oct 10, 2019·Acta Physiologica·Eva LasičMatjaž Stenovec
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan

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