PMID: 2123405Nov 12, 1990Paper

Dual effects of staurosporine on arachidonic acid metabolism in rat peritoneal macrophages

Biochimica Et Biophysica Acta
M WatanabeK Ohuchi

Abstract

Staurosporine is a microbial anti-fungal alkaloid having a most potent inhibitory activity on protein kinase C and is recently found as a non-12-O-tetradecanoylphorbol-13-acetate (non-TPA)-type tumor promoter of mouse skin, although tumor promotion induced by a TPA-type tumor promoter teleocidin is suppressed by staurosporine. When rat peritoneal macrophages were incubated in the medium containing various concentrations of staurosporine, prostaglandin E2 production and release of radioactivity from [3H]arachidonic acid-labeled macrophages were stimulated at concentrations of 1 and 10 ng/ml. But higher concentrations of staurosporine such as 100 and 1000 ng/ml showed no stimulative effect on prostaglandin E2 production although cytoplasmic free calcium levels were increased in a dose-dependent manner. Staurosporine-induced stimulation of prostaglandin E2 production was inhibited by treatment with cycloheximide, suggesting that a certain protein synthesis is prerequisite for the stimulation of arahcidonic acid metabolism. At higher concentrations (100 and 1000 ng/ml), staurosporine inhibited TPA-type tumor promoter (TPA, teleocidin and aplysiatoxin)-induced stimulation of arachidonic acid metabolism probably due to the inhibition...Continue Reading

References

Jan 1, 1987·Advances in Cancer Research·H Fujiki, T Sugimura
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M SuganumaT Sugimura
Oct 1, 1988·Japanese Journal of Cancer Research : Gann·H FujikiT Yasumoto
Dec 15, 1988·Biochemical and Biophysical Research Communications·K TakishimaM R Rosner
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J E NiedelG R Vandenbark
Apr 1, 1983·Environmental Health Perspectives·H FujikiR E Moore

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.