PMID: 2511851Nov 1, 1989Paper

Dual effects of thyrotrophin-releasing hormone (TRH) on K+ conductance in frog pituitary melanotrophs. TRH-induced alpha-melanocyte-stimulating hormone release is not mediated through voltage-sensitive K+ channels

Journal of Molecular Endocrinology
E LouisetH Vaudry

Abstract

Modulation of the activity of K+ channels by TRH and the possible involvement of this modulation in TRH-induced release of alpha-MSH were studied in cultured frog melanotrophs, using patch-clamp and perifusion techniques. Pars intermedia cells were enzymatically dispersed and cultured in Leibovitz medium. In order to test the viability of cultured cells, the amount of alpha-MSH released into the medium was measured by radioimmunoassay every day for 1 week of culture. The total amount of alpha-MSH released during the first 4 days of culture was 8.6 times higher than the intracellular content of alpha-MSH on day 1. Melanotrophs were identified by an indirect immunofluorescence technique using a specific antiserum to alpha-MSH. Recordings obtained in whole-cell, cell-attached and excised patch-clamp configurations showed that TRH induced a transient polarization concomitant with an increase in the probability of opening of Ca2+-activated K+ channels. This transient response was followed by a depolarization accompanied by an enhanced frequency of action potential discharge. TRH also induced a decrease in voltage-dependent K+ conductance. Application of tetraethylammonium, a K+ channel blocker, depolarized the cells and increased th...Continue Reading

Citations

Feb 1, 1994·Journal of Neuroendocrinology·E LouisetL Cazin
Jun 1, 1991·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·M LamaczH Vaudry
May 31, 1993·Annals of the New York Academy of Sciences·M C TononH Vaudry
May 14, 2009·General and Comparative Endocrinology·Ludovic GalasHubert Vaudry

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