Dual function for U2AF(35) in AG-dependent pre-mRNA splicing.

Molecular and Cellular Biology
Sabine GuthJuan Valcarcel

Abstract

The splicing factor U2AF is required for the recruitment of U2 small nuclear RNP to pre-mRNAs in higher eukaryotes. The 65-kDa subunit of U2AF (U2AF(65)) binds to the polypyrimidine (Py) tract preceding the 3' splice site, while the 35-kDa subunit (U2AF(35)) contacts the conserved AG dinucleotide at the 3' end of the intron. It has been shown that the interaction between U2AF(35) and the 3' splice site AG can stabilize U2AF(65) binding to weak Py tracts characteristic of so-called AG-dependent pre-mRNAs. U2AF(35) has also been implicated in arginine-serine (RS) domain-mediated bridging interactions with splicing factors of the SR protein family bound to exonic splicing enhancers (ESE), and these interactions can also stabilize U2AF(65) binding. Complementation of the splicing activity of nuclear extracts depleted of U2AF by chromatography in oligo(dT)-cellulose requires, for some pre-mRNAs, only the presence of U2AF(65). In contrast, splicing of a mouse immunoglobulin M (IgM) M1-M2 pre-mRNA requires both U2AF subunits. In this report we have investigated the sequence elements (e.g., Py tract strength, 3' splice site AG, ESE) responsible for the U2AF(35) dependence of IgM. The results indicate that (i) the IgM substrate is an AG...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P D Zamore, M R Green
Oct 1, 1989·Genes & Development·J Wu, J L Manley
Oct 1, 1989·Genes & Development·Y Zhuang, A M Weiner
Oct 1, 1989·Genes & Development·K K Nelson, M R Green
Aug 25, 1989·Nucleic Acids Research·A HemsleyD J Galas
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y A ZhuangA M Weiner
Sep 1, 1988·Genes & Development·A Krämer
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·G LazarH M Goodman
Jul 15, 1994·Genes & Development·M Tian, T Maniatis
Mar 1, 1993·Genes & Development·A WatakabeY Shimura
Jul 1, 1996·Genes & Development·J L Manley, R Tacke
Aug 1, 1996·Trends in Biochemical Sciences·J Valcárcel, M R Green
Jan 1, 1996·Annual Review of Biochemistry·A Krämer
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Z RudnerD C Rio
Jul 22, 1998·Molecular and Cellular Biology·O GozaniR Reed
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·A G EldridgeB J Blencowe
Jul 8, 1999·Current Opinion in Cell Biology·R Tacke, J L Manley
Nov 5, 1999·Methods in Molecular Biology·T A Cooper
Dec 22, 1999·RNA·P S Page-McCawP A Sharp
May 10, 2000·Current Opinion in Cell Biology·R Reed

❮ Previous
Next ❯

Citations

Jul 16, 2010·Human Genetics·Jana Kralovicova, Igor Vorechovsky
Jun 14, 2005·Biochemical and Biophysical Research Communications·Jun OkudaChihiro Sasakawa
Jul 3, 2013·Nature Communications·Marina N Nedelcheva-VelevaStoyno S Stoynov
Jul 21, 2006·Molecular Biology of the Cell·Teresa Raquel PachecoMaria Carmo-Fonseca
Oct 25, 2003·Nucleic Acids Research·Haidong Gu, Daniel R Schoenberg
Sep 6, 2005·Nucleic Acids Research·Jana KrálovicováIgor Vorechovský
Jan 13, 2006·Plant Physiology·Bing-Bing Wang, Volker Brendel
Aug 31, 2006·Molecular and Cellular Biology·Teresa R PachecoMaria Carmo-Fonseca
May 4, 2004·Molecular and Cellular Biology·Christopher J Webb, Jo Ann Wise
Oct 18, 2005·Molecular and Cellular Biology·T Nicolai SiegelGeorge A M Cross
Jun 21, 2007·PloS One·Michelle L HastingsAdrian R Krainer
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leigh A SkordisFrancesco Muntoni
Jul 19, 2012·Wiley Interdisciplinary Reviews. RNA·Jorge Pérez-Valle, Josep Vilardell
Mar 22, 2005·Biochimica Et Biophysica Acta·Kristy L HenscheidJ Andrew Berglund
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anant A AgrawalClara L Kielkopf
Oct 15, 2008·Molecular Cell·Sophie BonnalJuan Valcárcel
Oct 12, 2004·Experimental Cell Research·Hiroshi MaitaSanae M M Iguchi-Ariga
Oct 1, 2014·Genome Research·Janine O IlaganRobert K Bradley
Sep 26, 2002·FEBS Letters·Esther KellenbergerMichael Sattler
Aug 27, 2014·Wiley Interdisciplinary Reviews. RNA·Jonathan M Howard, Jeremy R Sanford
Jul 5, 2006·Genes & Development·José-María Izquierdo, Juan Valcárcel
Apr 21, 2004·The Journal of Biological Chemistry·Teresa R PachecoMaria Carmo-Fonseca
Sep 23, 2020·Nature Communications·Hisashi YoshidaEiji Obayashi
Sep 28, 2017·Wiley Interdisciplinary Reviews. RNA·Kinji OhnoAkio Masuda
May 19, 2005·The Journal of Biological Chemistry·Martin LützelbergerGöran Akusjärvi
Feb 10, 2021·Biochemical and Biophysical Research Communications·Bo ZhaoYouzhong Wan
Mar 11, 2021·Cell Chemical Biology·Rakesh ChatrikhiClara L Kielkopf
Oct 18, 2005·Gene·Magnus Wang, Antonio Marín
Aug 19, 2006·Veterinary Immunology and Immunopathology·Yi-Hsin FanHappy K Shieh
Jul 1, 2006·Science·Tracy L Kress, Christine Guthrie
Feb 14, 2012·Molecular Cell·Joao Paulo TavanezJuan Valcárcel
Jul 29, 2015·Genes & Development·Hisashi YoshidaEiji Obayashi

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