Dual Functional MicroRNA-186-5p Targets both FGF2 and RelA to Suppress Tumorigenesis of Glioblastoma Multiforme

Cellular and Molecular Neurobiology
Fachen WangBing Chen

Abstract

Glioblastoma multiforme (GBM) is one of the most malignant cancers. MicroRNAs (miRs) were reported to play important roles in GBM recently. However, the role of a novel miR-186-5p in GBM tumorigenesis is still elusive. Using bioinformatics, miR-186-5p was identified as potential regulators of both fibroblast growth factor (FGF)-2 and NF-κB subunit RelA. Luciferase reporter assay was used to confirm the direct recognition FGF2 and RelA mRNAs by miR-186-5p. Invasion and migration assays were employed to study the effect of miR-186-5p on GBM cell growth in vitro. Xenograft tumor animal model was established to elucidate the in vivo function of miR-186-5p. MiR-186-5p directly targeted mRNAs of both FGF2 and RelA, and repressed their expressions. Invasive and migratory abilities of GBM cells and growth of xenograft tumors were significantly inhibited by miR-186-5p, which can be restored by re-introduction of FGF2 and RelA expressions. MiR-186-5p is a novel tumor suppressor miR that functions to inhibit tumorigenesis of GBM both in vitro and in vivo, by targeting both FGF2 and RelA. MiR-186-5p/FGF2/RelA pathway may be potentially used as molecular targets of in the clinical treatment of GBM.

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Citations

May 12, 2018·Bioscience Reports·Lifeng XiaoYuru Li
Jul 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cai ZhangYanting Gu
Apr 5, 2019·Cell Proliferation·Xiaodiao KangZhi-Wei Wang
Mar 21, 2020·Frontiers in Oncology·Ying XiangShuai-Shuai Niu

Methods Mentioned

BETA
transfection
Protein Assay
xenograft
PCR
xenografts

Related Concepts

RELA protein, human
MIRN186 microRNA, human
Metazoa
Brain Tumor, Recurrent
Glioblastoma Multiforme
Mice, Inbred BALB C
Mice, Nude
Metastasis Suppressor Genes
Fibroblast Growth Factor 2
Xenograft Model Antitumor Assays

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