PMID: 7033564Dec 1, 1981Paper

Dual functions of bacteriophage T4D gene 28 product: structural component of the viral tail baseplate central plug and cleavage enzyme for folyl polyglutamates. II. Folate metabolism and polyglutamate cleavage activity of uninfected and infected Escherichia coli cells and bacteriophage

Journal of Virology
L M Kozloff, M Lute

Abstract

We investigated the role of the T4D bacteriophage gene 28 product in folate metabolism in infected Escherichia coli cells by using antifolate drugs and a newly devised assay for folyl polyglutamate cleavage activity. Preincubation of host E. coli cells with various sulfa drugs inhibited phage production by decreasing the burst size when the phage particles produced an altered gene 28 product (i.e., after infection under permissive conditions with T4D 28(ts) or T4D am28). In addition, we found that another folate analog, pyrimethamine, also inhibited T4D 28(ts) production and T4D 28am production, but this analog did not inhibit wild-type T4D production. A temperature-resistant revertant of T4D 28(ts) was not sensitive to either sulfa drugs or pyrimethamine. We developed an assay to measure the enzymatic cleavage of folyl polyglutamates. The high-molecular-weight folyl polyglutamate substrate was isolated from E. coli B cells infected with T4D am28 in the presence of labeled glutamic acid and was characterized as a folate compound containing 12 to 14 labeled glutamate residues. Extracts of uninfected bacteria liberated glutamate residues from this substrate with a pH optimum of 8.4 to 8.5. Extracts of bacteriophage T4D-infected E...Continue Reading

References

May 3, 1978·Biochimica Et Biophysica Acta·K Nakamura, L M Kozloff
Jun 1, 1970·Journal of Virology·L M KozloffL K Crosby
Jun 1, 1970·Journal of Virology·L M KozloffS S DeLong
Jul 1, 1965·Journal of Molecular Biology·L M Kozloff, M Lute
May 1, 1970·Analytical Biochemistry·C L Krumdieck, C M Baugh
Apr 1, 1980·Journal of Virology·S H Kao, W H McClain
Apr 1, 1980·Journal of Virology·S H Kao, W H McClain

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Citations

Jan 1, 1995·DNA Sequence : the Journal of DNA Sequencing and Mapping·R BovaA Storlazzi
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Nov 15, 1983·Biochemical and Biophysical Research Communications·D A Sadewasser, L M Kozloff

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