Dual inhibition of PCDH9 expression by miR-215-5p up-regulation in gliomas

Chunlin WangJun Zhong


The clinical prognosis of malignant gliomas is poor and PCDH9 down-regulation is strongly associated with its poor prognosis. But the mechanism of PCDH9 down-regulation is unknown. Abnormal miRNAs profiles regulate tumor phenotypes through inhibiting their target genes and miRNAs could inhibit target genes more efficiently by binding to both the promoter and 3'UTR of target genes. In this study, to search the dual inhibitory miRNAs which suppress PCDH9 expression in gliomas, we performed an integrative analysis of databases including miRDB, TargetScan, microPIR and miRCancer. We identified three candidate miRNAs which were predicted to bind both the promoter and 3'UTR of PCDH9 and up-regulated in gliomas. Then, we validated miR-215-5p up-regulation and PCDH9 down-regulation in glioma samples and demonstrated that miR-215-5p could inhibit the mRNA and protein levels of PCDH9 in glioma cell lines by targeting its promoter and 3' UTR at the same time. Moreover, miR-215-5p could increase glioma cell proliferation, clone formation, in-vitro migration and reduce apoptosis via inhibiting PCDH9 expression. Our study provides evidence for a novel dual inhibition of PCDH9 by miR-215-5p in gliomas and suggests that miR-215-5p might be a t...Continue Reading


Jan 11, 2001·The New England Journal of Medicine·L M DeAngelis
Jun 10, 2005·Nature·Jun LuTodd R Golub
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Aug 28, 2007·International Journal of Cancer. Journal International Du Cancer·Yasushi ShintaniKeith R Johnson
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert F PlaceRajvir Dahiya
Aug 2, 2008·The New England Journal of Medicine·Patrick Y Wen, Santosh Kesari
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel H KimJohn J Rossi
Oct 31, 2009·The AAPS Journal·Chunsheng LiLin Zhang
Apr 8, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stuart A GrossmanUNKNOWN NABTT CNS Consortium
Mar 24, 2011·Nucleic Acids Research·Scott T Younger, David R Corey
Feb 4, 2012·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Chunlin WangZhenshan Huang
Mar 23, 2012·PloS One·Jittima PiriyapongsaSissades Tongsima
Mar 29, 2013·The New England Journal of Medicine·Harry L A JanssenMichael R Hodges
Aug 29, 2013·Cancer Letters·Yujie DengTianhua Zhou
Nov 12, 2013·Journal of Molecular Neuroscience : MN·Chunlin WangJiachuan Liu
Jul 2, 2014·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y J Xu, Y Fan
Aug 31, 2014·Biochemical and Biophysical Research Communications·Pengfei ZhuFengmin Lu
Nov 8, 2014·Nucleic Acids Research·Nathan Wong, Xiaowei Wang
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Mar 2, 2016·Biochimica Et Biophysica Acta·Lingling MiaoYizheng Wang

❮ Previous
Next ❯


May 12, 2018·Yonsei Medical Journal·Hui Tian, Zhenkun He
Dec 19, 2017·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O I KitS B Panina
Feb 24, 2018·Molecular Cancer·Hongyu LiuYongguang Tao
Dec 18, 2020·Frontiers in Oncology·Tiziana AnneseDomenico Ribatti
Feb 13, 2021·Archives of Gynecology and Obstetrics·Rodolfo R FavaroTelma M T Zorn
Jul 3, 2021·International Journal of Molecular Sciences·Yinshan BaiJulang Li

❮ Previous
Next ❯

Methods Mentioned


Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.