Dual-label quantum dot-based immunoassay for simultaneous determination of Carbadox and Olaquindox metabolites in animal tissues

Food Chemistry
Tao LeHuan Yu

Abstract

A novel and reliable dual-label direct competitive fluorescence-linked immunosorbent assay (dc-FLISA) based on quantum dots (QDs) was developed for the simultaneous determination of the major metabolites of Carbadox and Olaquindox residues in animal tissues, using anti-QCA monoclonal antibodies and anti-MQCA polyclonal antibodies labeled with QD520 and QD635, respectively. The limits of detection for QCA and MQCA were 0.05 and 0.07ng/ml, respectively. The method was used to analyze fortified samples and analyte recoveries ranged from 81.5% to 98.2% (QCA) and 84.2% to 95.7% (MQCA). Good correlations between the dc-FLISA method and HPLC were demonstrated for the determination of QCA and MQCA residues in swine tissue samples, confirming the reliability of the proposed method.

References

Mar 1, 1995·Food Additives and Contaminants·M D RoseJ A Tarbin
Oct 8, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Masakazu Horie, Mitsunori Murayama
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M J HutchinsonD G Kennedy
Dec 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yanling ZhangZonghui Yuan
Oct 8, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lingli HuangZonghui Yuan
Apr 20, 2011·Analytica Chimica Acta·Carolina Carrillo-CarriónMiguel Valcárcel
Aug 11, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·D PengZ Yuan
Aug 13, 2011·Journal of Agricultural and Food Chemistry·Chunmei SongGaiping Zhang
Mar 20, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·T LeY Chen
Jan 21, 2014·Journal of Agricultural and Food Chemistry·Jenifer García-FernándezAlfredo Sanz-Medel

❮ Previous
Next ❯

Citations

Apr 4, 2019·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Tao PengHaiyang Jiang
Jun 23, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xulin WanXiaoqi Tao
Sep 22, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongwei ZhangBoyang Yu
Sep 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·I V MartynenkoY K Gun'ko
Sep 19, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Jiadi SunXiulan Sun
Mar 6, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Yankai LiuAiping Wang
Mar 31, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Aiping WangGaiping Zhang
Jan 30, 2018·Journal of Agricultural and Food Chemistry·Jia-Wei ZhouGuan-Hua Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.