PMID: 374585Jan 1, 1979Paper

Dual-laser flow cytometry of single mammalian cells

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J A SteinkampH A Crissman

Abstract

An improved dual-laser flow cytometric system for quantitative analysis and sorting of mammalian cells has been developed using a low-power argon and high-power krypton laser as illumination sources, thus permitting the excitation of fluorescent dyes having absorption regions ranging from the ultraviolet to infrared. Cells stained in liquid suspension with fluorescent dyes enter a flow chamber where they intersect two spatially separated laser beams. Separate pairs of quartz beam-shaping optics focus each beam onto the cell stream. Electro-optical sensors measure fluorescence and light scatter signals from cells that are processed electronically and displayed as frequency distribution histograms. Cells also can be electronically separated and microscopically identified. The ease and versatility of operation designed into this system represent a marked technological improvement for dual-laser excited flow systems. Details of this instrument are described along with illustrative examples of cells stained with mithramycin and rhodamine and analyzed for DNA content, total protein, and nuclear and cytoplasmic diameter.

Citations

Jan 25, 2012·Cytotechnology·Julien PicotChantal M Boulanger
Apr 6, 2012·Trends in Immunology·Sean C BendallPratip K Chattopadhyay
Nov 1, 1987·Experimental Cell Research·H A Crissman, J A Steinkamp
Jul 1, 1980·IEEE Transactions on Bio-medical Engineering·M R Groves
Oct 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Orlicky, S K Nordeen
Jan 1, 1982·Cytometry·C C Stewart, J A Steinkamp
Sep 1, 1980·Cytometry·D J Arndt-JovinT M Jovin
Dec 1, 1982·Journal of Cellular Physiology·Z DarzynkiewiczJ Steinkamp
Jan 1, 1981·Cytometry·W G Eisert, M Dennenloehr
Mar 10, 2020·Current Protocols in Cytometry·Laura Ferrer-FontKylie M Price
Sep 15, 1982·Analytical Biochemistry·H S Kruth
Feb 5, 2000·Analytical Chemistry·Z ZhangN J Dovichi

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