PMID: 9450962Apr 4, 1998Paper

Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles

Molecular Biology of the Cell
Regina LeberG Daum

Abstract

Squalene epoxidase, encoded by the ERG1 gene in yeast, is a key enzyme of sterol biosynthesis. Analysis of subcellular fractions revealed that squalene epoxidase was present in the microsomal fraction (30,000 x g) and also cofractionated with lipid particles. A dual localization of Erg1p was confirmed by immunofluorescence microscopy. On the basis of the distribution of marker proteins, 62% of cellular Erg1p could be assigned to the endoplasmic reticulum and 38% to lipid particles in late logarithmic-phase cells. In contrast, sterol Delta24-methyltransferase (Erg6p), an enzyme catalyzing a late step in sterol biosynthesis, was found mainly in lipid particles cofractionating with triacylglycerols and steryl esters. The relative distribution of Erg1p between the endoplasmic reticulum and lipid particles changes during growth. Squalene epoxidase (Erg1p) was absent in an erg1 disruptant strain and was induced fivefold in lipid particles and in the endoplasmic reticulum when the ERG1 gene was overexpressed from a multicopy plasmid. The amount of squalene epoxidase in both compartments was also induced approximately fivefold by treatment of yeast cells with terbinafine, an inhibitor of the fungal squalene epoxidase. In contrast to th...Continue Reading

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Citations

Feb 24, 2009·Applied Microbiology and Biotechnology·Wenliang ZhangQi Jin
Jun 14, 2005·Biochimica Et Biophysica Acta·René G OttGünther Daum
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·F BeaudoinJ A Napier
Dec 22, 1999·European Journal of Biochemistry·P Bouvier-NavéH Schaller
Feb 15, 2000·European Journal of Biochemistry·D ZweytickG Daum
Mar 17, 2010·The Journal of Biological Chemistry·Sepp D Kohlwein
Oct 18, 2006·Antimicrobial Agents and Chemotherapy·Christoph RuckenstuhlFriederike Turnowsky
Nov 10, 2009·Eukaryotic Cell·Tiffany M JoffrionMelanie T Cushion
Dec 1, 2012·Journal of Lipid Research·Ana Stevanovic, Christoph Thiele
Aug 22, 2009·Journal of Lipid Research·Joel M Goodman
Nov 10, 2010·PloS One·Bruno L Bozaquel-MoraisMónica Montero-Lomeli
Dec 24, 2011·PloS One·Fernando Alvarez-VasquezEberhard O Voit
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·C MoM Bard
Nov 20, 2013·Biochimica Et Biophysica Acta·Lisa KlugGünther Daum
Dec 25, 2009·The Journal of Biological Chemistry·Miroslava SpanovaGünther Daum
Jul 13, 2005·Applied Microbiology and Biotechnology·Megumi ShobayashiHaruyuki Iefuji
Dec 30, 2015·Biochimica Et Biophysica Acta·Chao-Wen Wang
Feb 9, 2008·Progress in Lipid Research·Sona RajakumariGünther Daum
Sep 4, 2007·Trends in Cell Biology·Michael A Welte
Aug 22, 2007·Progress in Lipid Research·William A Prinz
Apr 17, 2007·Biochimica Et Biophysica Acta·Timothy A Schulz, William A Prinz
Feb 28, 2007·Archives of Biochemistry and Biophysics·Lokendra Kumar SharmaRakesh Tuli
Jan 22, 2005·Molecular Microbiology·Marc WältermannAlexander Steinbüchel
Jul 7, 2011·Biology of the Cell·Ivan HapalaThierry Ferreira
Sep 28, 2012·The FEBS Journal·Minh T TaHongyuan Yang
Aug 28, 2004·Plant Physiology and Biochemistry : PPB·Li-Jen Lin, Jason T C Tzen
Aug 19, 2006·Biochimica Et Biophysica Acta·Tibor CzabanyGünther Daum
Aug 22, 2006·Biochimica Et Biophysica Acta·Maria L GasparSusan A Henry
Dec 21, 2013·BMB Reports·Yingmei LiuPingsheng Liu
May 20, 2015·Current Opinion in Cell Biology·Antonio Daniel BarbosaSymeon Siniossoglou
Apr 22, 2015·Cellular and Molecular Life Sciences : CMLS·Chao-Wen Wang

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