PMID: 2502402Jul 15, 1989Paper

Dual Mg2+ control of formyl-peptide-receptor--G-protein interaction in HL 60 cells. Evidence that the low-agonist-affinity receptor interacts with and activates the G-protein

European Journal of Biochemistry
P GierschikK H Jakobs

Abstract

In neutrophils and several other phagocytic cell types, a pertussis- and cholera-toxin-sensitive form of the guanine-nucleotide-binding protein (G-protein) Gp couples receptors for N-formylmethionine-containing chemotactic peptides to stimulation of phospholipase C. Using membranes of myeloid differentiated HL 60 cells, we have examined the role of Mg2+ and guanine nucleotides in regulating (a) the interaction of the formyl-peptide receptor with the chemotactic agonist N-formylmethionyl-leucyl-phenylalanine (fMet-Leu-Phe) and (b) the receptor-mediated activation of Gp. Mg2+ markedly enhanced the number of receptors with high affinity for the radiolabeled oligopeptide fMet-Leu-[3H]Phe. At the same time, Mg2+ largely increased the potency of guanosine-5'-(3-O-thio)triphosphate, but not of GDP or guanosine-5'-(2-O-thio)diphosphate, to inhibit binding of the peptide. Comparison of the potency of Mg2+ in eliciting these two effects and analysis of the specificities of the relevant divalent cation sites revealed that Mg2+ interacts with at least two independent sites on the receptor-Gp complex. One site is specific for Mg2+ and exhibits affinity in the micromolar range, the other site interacts with millimolar concentrations of sever...Continue Reading

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Citations

Jun 5, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Patrick KleemannRoland Seifert
Jan 1, 1996·General Pharmacology·J F KlinkerR Seifert
Mar 15, 1994·European Journal of Pharmacology·N FraeymanP Vanscheeuwijck
Aug 28, 2003·Life Sciences·Roland Seifert, Katharina Wenzel-Seifert
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Nov 20, 1989·European Journal of Biochemistry·E HerrmannK H Jakobs
Jun 15, 1992·European Journal of Biochemistry·M CampsP Gierschik
Sep 15, 1993·European Journal of Biochemistry·E LigetiK H Jakobs
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Nov 24, 1999·The Journal of Biological Chemistry·K Wenzel-SeifertR Seifert
Sep 3, 1998·The Journal of Biological Chemistry·K Wenzel-SeifertR Seifert
May 19, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Klaus AktoriesThomas Wieland
Sep 6, 2008·The Journal of Pharmacology and Experimental Therapeutics·Nina Weitl, Roland Seifert
Oct 5, 2001·Biochemical Pharmacology·F CeccarelliM R Mazzoni
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts

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