Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease

International Journal of Nanomedicine
Yabing WangChen Huang

Abstract

Combined therapies utilizing inhibitors to remove pathogens are needed to suppress lipopolysaccharide (LPS)-induced periodontal disease. We prepared a novel, multi-agent delivery scaffold for periodontal treatment. In this study, we synthesized SP600125 (a JNK inhibitor) and SB203580 (a p38 inhibitor) drug-loaded poly(ethylene glycol)-block-caprolactone copolymer via dialysis method. The physical property of micelles was characterized through dynamic light scattering and transmission electron microscopy. The cell growth and LPS-induced MMP-2 and MMP-13 expression were evaluated through CCK-8, real-time PCR and Western blot assay. The release of SP600125 and SB203580 from different scaffolds was estimated. Microcomputed tomography and histology were used for evaluating the effect of the micelles-loaded nanofibers on the treatment of class II furcation defects in dogs. The drug was then successfully incorporated into gelatin fibers during electrospinning process. We confirmed that the micelles had spherical structure and an average particle size of 160 nm for SP600125-micelles (SP-Ms) and 150 nm for SB203580-micelles (SB-Ms). The nanofiber scaffold showed excellent encapsulation capability, in vitro drug-release behavior, and cel...Continue Reading

Citations

Jul 30, 2020·Canadian Journal of Physiology and Pharmacology·Dragana StanisicSuresh C Tyagi
Nov 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xingzi LanYadong Tang

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Methods Mentioned

BETA
dynamic light scattering
PCR
protein assay
fluorescence imaging

Software Mentioned

ImageJ

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