Dual-Modality Poly-l-histidine Nanoparticles to Deliver Peptide Nucleic Acids and Paclitaxel for In Vivo Cancer Therapy.

ACS Applied Materials & Interfaces
Aniket WahaneRaman Bahal

Abstract

Cationic polymeric nanoformulations have been explored to increase the transfection efficiency of small molecules and nucleic acid-based drugs. However, an excessive positive charge density often leads to severe cell and tissue-based toxicity that restricts the clinical translation of cationic polymeric nanoformulations. Herein, we investigate a series of cationic poly(lactic-co-glycolic acid) (PLGA)-histidine-based nanoformulations for enhanced cytoplasmic delivery with minimal toxicity. PLGA/poly-l-histidine nanoparticles show promising physico-biochemical features and transfection efficiency in a series of in vitro and cell culture-based studies. Further, the use of acetone/dichloromethane as a solvent mixture during the formulation process significantly improves the morphology and size distribution of PLGA/poly-l-histidine nanoparticles. PLGA/poly-l-histidine nanoformulations undergo clathrin-mediated endocytosis. A contrast-matched small-angle neutron scattering experiment confirmed poly-l-histidine's distribution on the PLGA nanoformulations. PLGA/poly-l-histidine formulations containing paclitaxel as a small molecule-based drug and peptide nucleic acids targeting microRNA-155 as nucleic acid analog are efficacious in in ...Continue Reading

References

Dec 24, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·D WangJ Samuel
May 29, 1999·Bioconjugate Chemistry·P Midoux, M Monsigny
May 29, 2003·The Journal of Cell Biology·Ivan R Nabi, Phuong U Le
Jul 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Aug 2, 2003·Biotechnology Letters·Wim MartinetMark M Kockx
Dec 30, 2003·Genes, Chromosomes & Cancer·Markus MetzlerArndt Borkhardt
Jan 24, 2004·Biomaterials·M N V Ravi KumarC M Lehr
Jul 26, 2005·The Journal of Pathology·Joost KluiverAnke van den Berg
Aug 23, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Steven R LittleRobert Langer
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Mar 2, 2006·Expert Opinion on Drug Delivery·Vijay I KulkarniRayasa R Murthy
Jul 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hongtao LvJie Yan
Jul 21, 2007·Nature Protocols·Manuel ZeitelhoferRalf Dahm
Sep 12, 2007·Journal of Pharmaceutical Sciences·Padma Malyala, Manmohan Singh
Jan 31, 2008·JAMA : the Journal of the American Medical Association·Aaron J SchetterCurtis C Harris
Feb 24, 2009·Biomaterials·Malgorzata S CartieraW Mark Saltzman
Feb 17, 2010·The Journal of Cell Biology·Mirkka KoivusaloSergio Grinstein
Mar 23, 2010·Advanced Drug Delivery Reviews·Vladimir Torchilin
Jul 6, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Moritz Beck-BroichsitterThomas Kissel
Mar 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rong LiuZhongwei Gu
Feb 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabienne DanhierVéronique Préat
May 23, 2012·Current Medicinal Chemistry·M M CardosoA C A Roque
Jun 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Imran A BabarFrank J Slack
Jun 28, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Sam MattiskeDavid F Callen
Aug 14, 2012·Chemical Society Reviews·Sangram Keshari SamalPeter Dubruel
Oct 9, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rachel J FieldsW Mark Saltzman
Nov 13, 2012·International Journal of Nanomedicine·Eleonore Fröhlich
Sep 3, 2013·Advanced Healthcare Materials·Pouyan E BoukanyL James Lee
Jun 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Chiranjeevi PeetlaVinod Labhasetwar
Aug 27, 2014·Advanced Healthcare Materials·Rachel J FieldsW Mark Saltzman
Nov 20, 2014·Nature·Christopher J ChengFrank J Slack
Jan 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anisha GuptaW Mark Saltzman
Mar 4, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Charlene M DawidczykPeter C Searson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.