Dual-Mode Superresolution Imaging Using Charge Transfer Dynamics in Semiconducting Polymer Dots.

Angewandte Chemie
Yifei JiangJason McNeill

Abstract

In a conjugated polymer-based single-particle heterojunction, stochastic fluctuations of the photogenerated hole population lead to spontaneous fluorescence switching. We found that 405 nm irradiation can induce charge recombination and activate the single-particle emission. Based on these phenomena, we developed a novel class of semiconducting polymer dots that can operate in two superresolution imaging modes. The spontaneous switching mode offers efficient imaging of large areas, with <10 nm localization precision, while the photoactivation/deactivation mode offers slower imaging, with further improved localization precision (ca. 1 nm), showing advantages in resolving small structures that require high spatial resolution. Superresolution imaging of microtubules and clathrin-coated pits was demonstrated, under both modes. The excellent localization precision and versatile imaging options provided by these nanoparticles offer clear advantages for imaging of various biological systems.

References

Aug 1, 1994·Physical Review Letters·M YanT M Miller
Jun 30, 2005·Journal of the American Chemical Society·Andre J GesquierePaul F Barbara
Mar 22, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Changfeng WuJason McNeill
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Jan 27, 2009·Nature Methods·Sean A McKinneyLoren L Looger
Feb 12, 2009·ACS Nano·Changfeng WuJason McNeill
Jan 13, 2010·Chemical Reviews·Tracey M Clarke, James R Durrant
Oct 12, 2010·Journal of the American Chemical Society·Changfeng WuDaniel T Chiu
Mar 8, 2011·Angewandte Chemie·Changfeng WuDaniel T Chiu
Jul 15, 2011·Journal of the American Chemical Society·Christopher A TrainaGuillermo C Bazan
Dec 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dylan T BurnetteBechara Kachar
Feb 9, 2012·Nano Letters·Jiangbo YuJason McNeill
Sep 11, 2012·Journal of the American Chemical Society·Robert SteyrleuthnerDieter Neher
Jan 12, 2013·Angewandte Chemie·Changfeng Wu, Daniel T Chiu
Dec 30, 2015·The Journal of Physical Chemistry Letters·Artem A BakulinEleonora Vella
Feb 4, 2016·Chemical Communications : Chem Comm·Xinzi ZhangElizabeth J Harbron
Nov 1, 2016·The Journal of Physical Chemistry Letters·Wenxiao WangChristy F Landes
Dec 29, 2016·Chemical Reviews·Yifei Jiang, Jason McNeill

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.