Abstract
Foliar uptake of nitrogen dioxide (NO2) is governed by its reactive absorption mechanism, by which NO2 molecules diffuse through cell wall layers and simultaneously react with apoplastic ascorbate to form nitrous acid, which freely diffuses across plasmalemma. However, whether free diffusion of nitrous acid is the sole mechanism of foliar uptake of NO2 remains unknown. The involvement of ammonia-inhibitable nitrite transporters in the foliar uptake of NO2, as reported in nitrite transport in Arabidopsis roots, is also unknown. In this study, we treated Arabidopsis thaliana leaves with methionine sulfoximine (MSX) to inhibit incorporation of ammonia into glutamate and exposed them to 4 ppm 15N-labeled NO2 for 4 h in light followed by quantification of total nitrogen, reduced nitrogen, and ammonia nitrogen derived from NO2 using mass spectrometry and capillary electrophoresis. The total nitrogen derived from NO2 in leaves without MSX treatment was 587.0 nmol NO2/g fresh weight, of which more than 65% was recovered as reduced nitrogen. In comparison, MSX treatment decreased the total nitrogen and reduced nitrogen derived from NO2 by half. Thus, half of the foliar uptake of NO2 is not attributable to passive diffusion of nitrous ac...Continue Reading
References
Jul 1, 1992·The Plant Journal : for Cell and Molecular Biology·H VaucheretM Caboche
Jan 1, 1966·Plant Physiology·O C Taylor, F M Eaton
Apr 2, 2003·Plant & Cell Physiology·Mamoru OkamotoAnthony D M Glass
Apr 6, 2004·Planta·Anja BuhtzJulia Kehr
Jan 1, 1994·Environmental Pollution·H Saxe
Jan 1, 1989·Environmental Pollution·R Sandhu, G Gupta
Feb 20, 2007·Nitric Oxide : Biology and Chemistry·A SamouilovV V Khramtsov
Jun 15, 2007·Plant & Cell Physiology·Miwa SugiuraMasaaki Takahashi
Jul 16, 2010·Plant Cell Reports·Yanbo Hu, Guangyu Sun
Jun 28, 2011·Archives of Biochemistry and Biophysics·Santiago SignorelliAna Denicola
Jan 1, 2008·Meat Science·Karl-Otto Honikel
Jun 15, 2013·The New Phytologist·Zorica KoturAnthony D M Glass
Apr 1, 1983·Planta·G Kaiser, U Heber
Dec 21, 2013·The New Phytologist·Misa TakahashiHiromichi Morikawa
Jul 1, 1990·The New Phytologist·Alan R Wellburn
Dec 1, 1993·The New Phytologist·P RamgeG H Kohlmaier