Dual over-expression pattern of membrane-type metalloproteinase-1 in cancer and stromal cells in human gastrointestinal carcinoma revealed by in situ hybridization and immunoelectron microscopy

International Journal of Cancer. Journal International Du Cancer
H OhtaniH Nagura

Abstract

Membrane-type metalloproteinase-I (MTI-MMP) is a transmembrane metalloproteinase, which activates pro-gelatinase A. There has been disagreement as to whether the cell types expressing MTI-MMP are cancer cells or stromal fibroblasts. Using human gastrointestinal carcinomas, the present study disclosed the tissue localization of MTI-MMP mRNA by in situ hybridization and ultrastructural localization of its protein by immunoelectron microscopy. In normal colon and stomach tissues, MTI-MMP mRNA and protein were negative or faintly positive both in epithelial cells and in stromal fibroblasts, except in the fundic gland of the stomach, which showed the positivity for MTI-MMP. In contrast, gastrointestinal cancer tissue showed over-expression of MTI-MMP mRNA and protein both in cancer cells and in stromal cells (fibroblasts). Stromal fibroblasts also expressed mRNA for gelatinase A and type-I procollagen. Double immunohistochemistry revealed that macrophages were also positive for MTI-MMP. Immunoelectron microscopy showed that MTI-MMP was localized along the plasma membrane of cancer cells and macrophages and in rough endoplasmic reticulum of fibroblasts. The present study reveals a dual over-expression pattern of MTI-MMP both in cance...Continue Reading

References

Aug 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H OhtaniH Nagura
Sep 15, 1995·International Journal of Cancer. Journal International Du Cancer·H OhtaniH Nagura
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A OkadaP Basset
Jan 1, 1993·Annual Review of Cell Biology·W G Stetler-StevensonL A Liotta
Jun 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F UeharaT Muramatsu
Jan 1, 1993·Breast Cancer Research and Treatment·K TryggvasonC Pyke
Jan 3, 1996·International Journal of Cancer. Journal International Du Cancer·B S NielsenK Danø
Jan 17, 1996·International Journal of Cancer. Journal International Du Cancer·C GillesJ M Foidart

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Citations

Dec 5, 1998·Japanese Journal of Clinical Oncology·H NakayamaM Hiroi
Jan 30, 2013·Biochemical and Biophysical Research Communications·Ju Hee LeeJeong-Heon Ko
Nov 27, 2002·Clinical Colorectal Cancer·Angela G Mysliwiec, Deborah L Ornstein
May 18, 1999·Biochemical and Biophysical Research Communications·S Hasan, M Nakajima
Jul 3, 1999·Japanese Journal of Cancer Research : Gann·S IshigakiM Seiki
Sep 6, 2000·The British Journal of Surgery·E A BakerD J Leaper
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M Seiki
Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·K KoR Takahashi
Jun 24, 2008·Journal of Toxicology and Environmental Health. Part a·Masashi KatoYuji Goto
Oct 20, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Motoji OkiTooru Shimosegawa
Jan 6, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Noriko OhtaniOsamu Yoshie
Feb 21, 2008·Clinical & Experimental Metastasis·Lidiya S Orlichenko, Derek C Radisky
Jan 7, 2010·The American Journal of Dermatopathology·Hoda RasheedSoheir Mahfouz
Jul 17, 1999·British Journal of Cancer·R S Boyd, F R Balkwill
Mar 25, 2000·Diseases of the Colon and Rectum·T C SardinhaS Abramson
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N E SounniA Noel
Aug 7, 2014·Clinical and Translational Medicine·Joseph W O'Connor, Esther W Gomez
Jan 6, 2000·Molecular Pathology : MP·E A GarbettN J Brown
Apr 19, 2003·Experimental Dermatology·E Kerkelä, U Saarialho-Kere
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M H KimR H Goldfarb
Mar 5, 1999·Breast Cancer Research and Treatment·M ToiT Tominaga
Dec 16, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y KatoK Miyazaki
Feb 17, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E KoblinskiB F Sloane

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