Dec 14, 2004

Dual oxidase2 is expressed all along the digestive tract

American Journal of Physiology. Gastrointestinal and Liver Physiology
Rabii Ameziane El HassaniCorinne Dupuy

Abstract

The dual oxidase (Duox)2 flavoprotein is strongly expressed in the thyroid gland, where it plays a critical role in the synthesis of thyroid hormones by providing thyroperoxidase with H2O2. DUOX2 mRNA was recently detected by RT-PCR and in-situ hybridization experiments in other tissues, such as rat colon and rat and human epithelial cells from the salivary excretory ducts and rectal glands. We examined Duox2 expression at the protein level throughout the porcine digestive tract and in human colon. Western blot analysis identified Duox2 as the same two molecular species (M(r) 165 and 175 kDa) as detected in the thyroid. It was expressed in all the tissues tested, but the highest levels were found in the cecum and sigmoidal colon. Immunohistochemical studies showed that Duox2 protein is mainly present in these parts of the gut and located at the apical membrane of the enterocytes in the brush border, indicating that it is expressed only in highly differentiated cells. A Ca2+/NADPH-dependent H2O2-generating system was associated with Duox2 protein expression, which had the same biochemical characteristics as the NADPH oxidase in the thyroid. Indeed, treatment of the thyroid and cecum particulate fractions with phenylarsine oxide ...Continue Reading

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Mentioned in this Paper

DUOX2 gene
Calcium [EPC]
Taenia Coli
Hydrogen Peroxide
Enzymes, antithrombotic
Calcium
Squamous Transitional Epithelial Cell Count
Oxidase
Duodenum
Oxophenylarsine

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