Dual regulation of the DeltaNp63 transcriptional activity by DeltaNp63 in human nasopharyngeal carcinoma cell

Biochemical and Biophysical Research Communications
Wing-Keung ChuJan-Kan Chen

Abstract

p63 splicing variants lacking NH(2)-terminal transactivating domain, known as DeltaNp63, are thought to antagonize p53 and p63 functions and are suggested to play roles in keratinocyte differentiation. Here, we show that DeltaNp63 has a dual-regulatory effect on the activity of its own promoter in NPC-076 cell. Down-regulation of the transcriptional activity is observed when DeltaNp63 is present in low levels. In contrast, up-regulation of DeltaNp63 transcriptional activity is observed when DeltaNp63 is expressed at higher levels. The down-regulation effect is abolished when the p53-binding site of the DeltaNp63 promoter is mutated. In sharp contrast, similar mutation does not affect the up-regulation of the DeltaNp63 transcriptional activity under the same experimental conditions. Further study shows that the up-regulation is correlated with the activation of the STAT3, as the blockade of STAT3 nuclear translocation abolishes the up-regulation by DeltaNp63. Thus, DeltaNp63 exerts a bidirectional regulation of the DeltaNp63 transcriptional activity in NPC-076 cell.

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Citations

Aug 25, 2009·Nucleic Acids Research·Marina MangiulliAnna Maria D'Erchia
Mar 29, 2018·Cellular & Molecular Biology Letters·Michaela GaloczovaBorivoj Vojtesek
Nov 2, 2017·Bioscience, Biotechnology, and Biochemistry·Jeng-Yuan Yao, Jan-Kan Chen
May 5, 2007·Expert Reviews in Molecular Medicine·Qian Tao, Anthony T C Chan
Jan 17, 2008·The Journal of Biological Chemistry·Wing-Keung ChuJan-Kan Chen
Oct 28, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuka OkadaChia-Yang Liu
Feb 28, 2021·The Journal of Pathology·Zuzana PokornáPhilip J Coates
Apr 26, 2011·Biochimica Et Biophysica Acta·Vincenzo Graziano, Vincenzo De Laurenzi

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