Dual RNA-Seq Analysis of Trichophyton rubrum and HaCat Keratinocyte Co-Culture Highlights Important Genes for Fungal-Host Interaction

Genes
Monise Fazolin PetrucelliAna Lúcia Fachin

Abstract

The dermatophyte Trichophyton rubrum is the major fungal pathogen of skin, hair, and nails that uses keratinized substrates as the primary nutrients during infection. Few strategies are available that permit a better understanding of the molecular mechanisms involved in the interaction of T. rubrum with the host because of the limitations of models mimicking this interaction. Dual RNA-seq is a powerful tool to unravel this complex interaction since it enables simultaneous evaluation of the transcriptome of two organisms. Using this technology in an in vitro model of co-culture, this study evaluated the transcriptional profile of genes involved in fungus-host interactions in 24 h. Our data demonstrated the induction of glyoxylate cycle genes, ERG6 and TERG_00916, which encodes a carboxylic acid transporter that may improve the assimilation of nutrients and fungal survival in the host. Furthermore, genes encoding keratinolytic proteases were also induced. In human keratinocytes (HaCat) cells, the SLC11A1, RNASE7, and CSF2 genes were induced and the products of these genes are known to have antimicrobial activity. In addition, the FLG and KRT1 genes involved in the epithelial barrier integrity were inhibited. This analysis showed ...Continue Reading

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Citations

Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Tunahan ÇakırSaliha Durmuş
Jul 15, 2020·Life Science Alliance·Brittany L UhlornSamuel K Campos
Feb 28, 2020·Oxidative Medicine and Cellular Longevity·Meiling HuangHongfeng Tang
Nov 27, 2020·Journal of Fungi·Monise Fazolin PetrucelliAna Lúcia Fachin
Jan 9, 2021·Cell Death & Disease·Xuan WangYunfang Wang
Oct 5, 2021·Frontiers in Cellular and Infection Microbiology·Pengxiu DaiXinke Zhang

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Datasets Mentioned

BETA
GSE110073

Methods Mentioned

BETA
RNA-seq
Assay
scraping
electrophoresis
PCR
RNAseq

Software Mentioned

DEseq
Enrichr enrichment tool
Bioconductor
R
BayGO
Genomic Ranges Bioconductor package
FastQC

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Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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