Dual RNAseq highlights the kinetics of skin microbiome and fish host responsiveness to bacterial infection.

Animal Microbiome
Jeremy Le LuyerD. Saulnier

Abstract

Tenacibaculum maritimum is a fish pathogen known for causing serious damage to a broad range of wild and farmed marine fish populations worldwide. The recently sequenced genome of T. maritimum strain NCIMB 2154T provided unprecedented information on the possible molecular mechanisms involved in the virulence of this species. However, little is known about the dynamic of infection in vivo, and information is lacking on both the intrinsic host response (gene expression) and its associated microbiota. Here, we applied complementary omic approaches, including dual RNAseq and 16S rRNA gene metabarcoding sequencing using Nanopore and short-read Illumina technologies to unravel the host-pathogen interplay in an experimental infection system using the tropical fish Platax orbicularis as model. We showed that the infection of the host is characterised by an enhancement of functions associated with antibiotic and glucans catabolism functions but a reduction of sulfate assimilation process in T. maritimum. The fish host concurrently displays a large panel of immune effectors, notably involving innate response and triggering acute inflammatory response. In addition, our results suggest that fish activate an adaptive immune response visible...Continue Reading

References

Jan 1, 1992·Neuroscience and Biobehavioral Reviews·E O JohnsonP W Gold
Aug 13, 1998·Immunological Reviews·W I WeisK Drickamer
Jul 23, 1999·Infection and Immunity·A Casadevall, L A Pirofski
Nov 7, 2001·Cellular Microbiology·M Kato-MaedaP M Small
Aug 27, 2002·Fish & Shellfish Immunology·M Claire H Holland, John D Lambris
Jan 22, 2004·Bioinformatics·Emmanuel ParadisKorbinian Strimmer
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·John F RawlsJeffrey I Gordon
Jun 14, 2006·Journal of Fish Diseases·R Avendaño-HerreraB Magariños
Oct 25, 2006·Diseases of Aquatic Organisms·Ruben Avendaño-HerreraBeatriz Magariños
Jul 9, 2008·Fish & Shellfish Immunology·Roy A Dalmo, Jarl Bøgwald
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Dec 17, 2009·Cell Research·Jinfang Zhu, William E Paul
Mar 20, 2010·Mucosal Immunology·T PérezJ L Múzquiz
Jul 24, 2010·British Journal of Pharmacology·Carol KilkennyUNKNOWN NC3Rs Reporting Guidelines Working Group
Mar 19, 2011·Developmental and Comparative Immunology·Yniv Palti
Jun 10, 2011·Bioinformatics·Petr DanecekUNKNOWN 1000 Genomes Project Analysis Group
Mar 14, 2012·Nature Reviews. Genetics·Ilseung Cho, Martin J Blaser
Apr 20, 2012·PloS One·Younes SmaniJerónimo Pachón
Jul 10, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Stephanie N ShishidoSherry D Fleming
Aug 15, 2012·Nature Reviews. Microbiology·Alexander J WestermannJörg Vogel
Dec 29, 2012·BMC Genomics·Brian J HaasJonathan Livny
May 21, 2013·Cell Host & Microbe·James E Cassat, Eric P Skaar
Sep 17, 2013·Fish & Shellfish Immunology·Tiehui Wang, Christopher J Secombes
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 9, 2015·Nature Communications·Hanna SusiAnna-Liisa Laine
Feb 6, 2015·Nature Communications·Melanie P MatheuMichael D Cahalan
May 21, 2015·Immunity·Ken Cadwell
Jul 15, 2015·Nature Reviews. Immunology·Tomas Ganz, Elizabeta Nemeth
Aug 4, 2015·Frontiers in Immunology·David ParraLluis Tort
Dec 18, 2015·Proceedings. Biological Sciences·Katja-Riikka LouhiAnssi Karvonen
Mar 29, 2016·Journal of Molecular Biology·Jacob E Choby, Eric P Skaar

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Software Mentioned

DADA2
ape R package
HTSeq
BBtools
DESeq2 R package
vegan R package
survival R package
count
phyloseq R package
MinKnow

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