Dual Role of 5-Lipoxygenase in Osteoclastogenesis in Bacterial-induced Apical Periodontitis

Journal of Endodontics
Francisco Wanderley Garcia Paula-SilvaLúcia Helena Faccioli

Abstract

The aim of this study was to evaluate the role of 5-lipoxigenase (5-LO) in the signaling for osteoclast formation and bone resorption in apical periodontitis (AP) after root canal contamination with oral bacteria. AP was experimentally induced in C57BL/6 mice because of contamination of the root canals left open to the oral environment. MK886 was used as a systemic inhibitor of 5-LO (5 mg/kg, daily). After 7, 14, 21, and 28 days, the animals were euthanized, and tissues were removed for gene evaluation by quantitative reverse transcriptase polymerase chain reaction, histologic analysis, and tartrate-resistant acid phosphatase staining. Root canal contamination induced the expression of messenger RNA for 5-LO and leukotriene B4 receptors BLT1 and BLT2. The administration of the 5-LO inhibitor reduced early receptor activator of nuclear factor kappa-B and receptor activator of nuclear factor kappa-B ligand synthesis but augmented late receptor activator of nuclear factor kappa-B ligand and osteoprotegerin expression during the course of AP development. Interestingly, long-term inhibition of 5-LO resulted in increased bone resorption and induced tartrate-resistant acid phosphatase-positive osteoclast formation. The divergent findi...Continue Reading

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Citations

Oct 12, 2016·Bioscience Trends·Yingping XuLing Wang
Nov 11, 2017·Journal of Dental Research·Y WuJ Wang
Jun 6, 2019·Journal of Applied Oral Science : Revista FOB·Fernanda Regina Ribeiro-SantosFrancisco Wanderley Garcia Paula-Silva
Apr 11, 2019·Brazilian Dental Journal·Sidinéia Feitoza de JesusLéa Assed Bezerra da Silva
May 14, 2020·Journal of Applied Oral Science : Revista FOB·Francisco Wanderley Garcia Paula-SilvaLúcia Helena Faccioli
Feb 15, 2020·Archives of Oral Biology·Francisco Wanderley Garcia Paula-SilvaLúcia Helena Faccioli
Apr 2, 2021·Clinical Oral Investigations·Fernanda Regina Ribeiro-SantosFrancisco Wanderley Garcia Paula-Silva
Jan 7, 2021·International Endodontic Journal·I B F PeteanF W G Paula-Silva

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