Dual role of IGF-II in oocyte maturation in southern flounder Paralichthys lethostigma: up-regulation of mPRα and resumption of meiosis

General and Comparative Endocrinology
Matthew E PichaPeter Thomas

Abstract

Increasing evidence suggests a regulatory role for the IGF system in teleost oocyte maturation (OM). Our objectives were to determine if IGF-I and IGF-II regulate different stages of OM in southern flounder (Paralichthys lethostigma) and to identify the likely maturation-inducing steroid (MIS) in this species. The most abundant final product of ovarian steroidogenesis assays eluted at the position of 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S). 20β-S was also more potent in inducing germinal vesicle breakdown (GVBD) of maturationally-competent oocytes than other teleost MISs. IGF-II (100 nM) induced maturational competence (OMC), as greater GVBD was induced after incubation with IGF-II+20β-S compared to that of the 20β-S+20β-S or IGF-II+no treatment group. Incubation with IGF-II (100 nM) for 4-8 h significantly increased ovarian membrane progestin receptor alpha (mPRα or Paqr7b) mRNA levels 12-15% and mPRα protein levels 75-101%. Further, the IGF-II-induced increase in mPRα protein concentrations was partially blocked by pretreatment with Wortmannin, a Pik3 inhibitor, and PD 098,059, a Mapk inhibitor. Both IGF-I and -II (100 nM) induced GVBD of maturationally-competent oocytes was blocked by incubation with cycloheximide. Incu...Continue Reading

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Citations

Jul 25, 2014·General and Comparative Endocrinology·Jean-François BaroillerManfred Reinecke
Oct 27, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Debabrata DasSudipta Maitra
Mar 5, 2013·General and Comparative Endocrinology·Pieter Van WielendaeleJozef Vanden Broeck
Feb 25, 2015·Zygote : the Biology of Gametes and Early Embryos·Sudip HajraSudipta Maitra
Jun 4, 2015·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Bao ShiKun Zang
Apr 16, 2020·Science China. Life Sciences·Tahir MuhammadZi-Jiang Chen
Mar 12, 2021·Biology of Reproduction·Jianzhen LiChristopher H K Cheng

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