Dual-Sensitive Hydrogel Nanoparticles Based on Conjugated Thermoresponsive Copolymers and Protein Filaments for Triggerable Drug Delivery

ACS Applied Materials & Interfaces
Roujin GhaffariAbdolreza Simchi

Abstract

In this study, novel hydrogel nanoparticles with dual triggerable release properties based on fibrous structural proteins (keratin) and thermoresponsive copolymers (Pluronic) are introduced. Nanoparticles were used for curcumin delivery as effective and safe anticancer agents, the hydrophobicity of which has limited their clinical applications. A drug was loaded into hydrogel nanoparticles by a single-step nanoprecipitation method. The drug-loaded nanoparticles had an average diameter of 165 and 66 nm at 25 and 37 °C, respectively. It was shown that the drug loading efficiency could be enhanced through crosslinking of the disulfide bonds in keratin. Crosslinking provided a targeted release profile under reductive conditions using an in vivo agent, glutathione (GSH), or in the presence of trypsin. Cytocompatibility assay using HeLa and L929 fibroblast cells exhibited no adverse effect of nanoparticles on cell viability up to 1 mg/mL. Besides, the green fluorescence of curcumin confirmed the uptake of drug-loaded nanoparticles by cancer cells. The redox and temperature-sensitive nanoparticles are potentially useable for the efficient delivery of hydrophobic drugs to targeted regions having a triggerable release profile.

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Citations

Jun 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xuexia LinShu-Feng Zhou
Feb 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mahshid Moballegh NaseryGautam Sethi
Mar 7, 2020·Journal of Biomedical Materials Research. Part a·Elnaz TamjidMaryam Nikkhah
Sep 2, 2020·Advanced Drug Delivery Reviews·Anastasia VarankoAshutosh Chilkoti
May 7, 2020·ACS Applied Materials & Interfaces·Giuseppe CavallaroYuri Lvov
May 19, 2021·International Journal of Biological Macromolecules·Linqi YunJianbin Li
May 16, 2021·International Journal of Biological Macromolecules·Mianhong ChenHaiming Cheng
May 26, 2021·Drug Development and Industrial Pharmacy·Na QiuGuangxi Zhai
Aug 30, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Piotr MazurekAnne L Skov

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