Dual signal transduction mechanisms modulate ciliary beat frequency in upper airway epithelium

The American Journal of Physiology
B YangT V McCaffrey

Abstract

This study investigated the effects of methacholine and terbutaline on the ciliary beat frequency (CBF) of upper airway epithelium. The CBF of cultured human adenoid explants was measured using microphotometry. Methacholine (10(-6) M) and terbutaline (10(-6)M) increased CBF a maximum of 23.0 +/- 1.8% (P < 0.001) and 16.5 +/- 2.3% (P < 0.001). Inhibition of endogenous nitric oxide (NO) production by nitro-L-arginine methyl ester (L-NAME) (10(-6) M) abolished the effects of methacholine in L-arginine-free medium (P < 0.008). This inhibition was reversed by addition of L-arginine. There was no inhibition of terbutaline-induced ciliostimulation by L-NAME (P < 0.5). KT-5823 (10(-6)M), a guanosine 3',5'-cyclic monophosphate (cGMP) kinase inhibitor, significantly inhibited the effects of methacholine (P < 0.0001), but not terbutaline (P > 0.15). H-89 (10(-6) M), a cAMP kinase inhibitor, significantly inhibited terbutaline-induced ciliostimulation (P < 0.0001), but not methacholine-induced ciliostimulation (P > 0.05). Diclofenac (10(-6) M), a cyclooxygenase inhibitor, significantly inhibited the effects of methacholine (P < 0.0007) but had no effect on terbutaline-induced ciliostimulation (P > 0.05). These findings suggest that the CBF...Continue Reading

Citations

Dec 21, 2000·American Journal of Physiology. Cell Physiology·O ZagooryZ Priel
Apr 19, 2003·American Journal of Respiratory and Critical Care Medicine·Maria DejaKlaus Lewandowski
Nov 3, 2006·American Journal of Respiratory Cell and Molecular Biology·Margaret K ElliottTodd A Wyatt
Mar 14, 2013·BioMed Research International·Claudia GonzálezManuel Villalón
May 17, 2002·Journal of Applied Physiology·Jeffrey I FrohockMatthias Salathe
Apr 5, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shandra A DoranJeffrey I Goldberg
Mar 6, 2015·Biological & Pharmaceutical Bulletin·Sveinbjörn Gizurarson
Nov 16, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Todd A WyattJoseph H Sisson

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