PMID: 9448030Feb 3, 1998Paper

Dual silver staining to characterise Helicobacter spp. outer membrane components

Journal of Immunological Methods
Jacqueline I KeenanP Bagshaw

Abstract

Helicobacter pylori is a bacterial pathogen, estimated to infect half the world's population. The bacterium is the aetiological cause of gastritis, the common precursor for peptic ulcer disease and gastric cancer. Immunisation of at-risk individuals is the most cost-effective means of dealing with such a widespread pathogen. Potential vaccine candidates need to be identified and characterised. Conventional silver staining is commonly used for the sensitive detection of bacterial protein components separated by SDS-PAGE. Modified silver stains employing periodate oxidation have also been developed for the analysis of purified bacterial lipopolysaccharide. By using these methods in parallel, as a dual silver stain, bacterial fractions can be characterised in terms of protein and LPS content. Strain differences can also be readily identified by comparing protein and LPS profiles. When combined with differential immunoblotting, the dual silver stain is a useful analytical tool for characterising potential vaccine candidate antigens.

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Citations

May 25, 2004·Antimicrobial Agents and Chemotherapy·Michael V BlandJacqueline I Keenan
Sep 30, 2010·Infection and Immunity·Heather ParkerJacqueline I Keenan
Sep 16, 2009·BMC Microbiology·Hideo YonezawaShigeru Kamiya
Apr 10, 2012·Analytical Biochemistry·Zhong-Xin ZhuXiao-Kun Li
Jul 26, 2015·FEMS Microbiology Letters·Wendong WangMintao Zhong
Jun 17, 2015·BioMed Research International·Hideo YonezawaShigeru Kamiya
Sep 14, 2011·Microbes and Infection·Heather Parker, Jacqueline I Keenan
Jun 3, 2021·International Journal of Molecular Sciences·María Fernanda GonzálezAndrew F G Quest
Sep 20, 2018·ACS Chemical Biology·Gloria AndolinaXuechen Li

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