Dual Stain With SATB2 and CK20/Villin Is Useful to Distinguish Colorectal Carcinomas From Other Tumors

American Journal of Clinical Pathology
Zaibo LiWendy L Frankel

Abstract

Small sample size limits the number of immunostains that may be attempted in colorectal carcinoma (CRC) biopsy specimens. We investigated the utility of dual stain with special AT-rich sequence binding protein 2 (SATB2) or caudal-type homeobox 2 (CDX2) and cytokeratin 20 (CK20) or villin in identifying CRC. Tissue microarrays with 222 CRCs and 375 other carcinomas were built. Dual stain was performed pairing nuclear stains CDX2 or SATB2 with CK20 or villin. All four single stains showed excellent sensitivity (93%-99%) but variable specificity (56%-88%) for CRC. All four dual stains also showed excellent sensitivity (90%-96%) while much improved specificity (88%-98%) compared with single stains. SATB2 dual stain (with CK20 or villin) showed a higher specificity than CDX2 dual stain (with CK20 or villin) with a comparable sensitivity. SATB2 dual stain shows the greatest potential clinical utility in identifying CRC and is superior to CDX2 dual stain. More important, SATB2 dual stain could be helpful for specimens with limited tissues or those having a nonclassic staining pattern.

References

Aug 14, 2003·Human Molecular Genetics·David R FitzPatrickDavid T Bonthron
Jun 19, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vassil KaimaktchievChristopher L Corless
Oct 7, 2004·Journal of the National Cancer Institute·Jessica B O'ConnellClifford Y Ko
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Jun 17, 2011·The American Journal of Surgical Pathology·Kristina MagnussonFredrik Pontén
Dec 20, 2011·World Journal of Gastrointestinal Surgery·Reda S SaadMei Xu
May 25, 2013·Histopathology·James R Conner, Jason L Hornick
Feb 28, 2015·The American Journal of Surgical Pathology·Alberto RighiAngelo Paolo Dei Tos
Mar 25, 2017·American Journal of Clinical Pathology·Zaibo LiWendy L Frankel

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