PMID: 6172144Dec 1, 1981Paper

Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction

The British Journal of Ophthalmology
M J Taylor, C J Hunt

Abstract

Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain alizarin red S provides a simple, quick technique for visualisation of both damaged and normal cells, thereby permitting the quantification of endothelial cell damage. Adjustment of the pH of the alizarin red S reagent to 4.2 is important for optimum dye-laking at the intercellular borders, and brief fixation with glutaraldehyde maintains the staining effect of both dyes.

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Citations

Feb 28, 1983·Documenta Ophthalmologica. Advances in Ophthalmology·J L van DelftA M Eijkelenboom
Apr 26, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christian MeltendorfJan Schroeter
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