Dual stimuli polysaccharide nanovesicles for conjugated and physically loaded doxorubicin delivery in breast cancer cells

Nanoscale
P S PramodM Jayakannan

Abstract

The present work reports the development of pH and enzyme dual responsive polysaccharide vesicular nano-scaffolds for the administration of doxorubicin via physical loading and polymer-drug conjugation to breast cancer cells. Dextran was suitably modified with a renewable resource 3-pentadecyl phenol unit through imine and aliphatic ester chemical linkages that acted as pH and esterase enzyme stimuli, respectively. These dual responsive polysaccharide derivatives self-organized into 200 ± 10 nm diameter nano-vesicles in water. The water soluble anticancer drug doxorubicin (DOX·HCl) was encapsulated in the hydrophilic pocket to produce core-loaded polysaccharide vesicles whereas chemical conjugation produced DOX anchored at the hydrophobic layer of the dextran nano-vesicles. In vitro studies revealed that about 70-80% of the drug was retained under circulatory conditions at pH = 7.4 and 37 °C. At a low pH of 6.0 to 5.0 and in the presence of esterase; both imine and ester linkages were cleaved instantaneously to release 100% of the loaded drugs. Cytotoxicity assays on Wild Type Mouse Embryonic Fibroblasts (WTMEFs) confirmed the non-toxicity of the newly developed dextran derivatives at up to 500 μg mL(-1) in PBS. MTT assays on f...Continue Reading

References

Dec 1, 1984·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J L Wike-HooleyH S Reinhold
Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·R Mehvar
Oct 27, 2005·Journal of the American Chemical Society·Miao YangGerhard Wegner
Dec 17, 2008·Biomacromolecules·Clément HougaJean-François Le Meins
Feb 28, 2009·Angewandte Chemie·Christophe SchatzSébastien Lecommandoux
Jan 23, 2010·Nature Materials·Martien A Cohen StuartSergiy Minko
Apr 1, 2010·Chemical Reviews·Jonathan P CelliTayyaba Hasan
Jul 28, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Xiaomeng ZhangRobert J Gillies
Sep 15, 2010·Molecular Pharmaceutics·Weiwei GaoOmid C Farokhzad
Feb 11, 2011·Angewandte Chemie·So Young ParkEun Seong Lee
Mar 8, 2011·Bioconjugate Chemistry·Bo ChenFrancis C Szoka
Apr 7, 2011·Chemical Communications : Chem Comm·Qi-Da HuJun Li
Oct 12, 2011·Journal of the American Chemical Society·Jin-Zhi DuJun Wang
Nov 17, 2011·Chemical Society Reviews·Shoshy Mizrahy, Dan Peer
Jun 19, 2012·Chemistry of Materials : a Publication of the American Chemical Society·Nate Larson, Hamidreza Ghandehari
Nov 6, 2012·Chemical Communications : Chem Comm·Jiangtao XuGreg G Qiao
Apr 25, 2013·Nanoscale·Adrian V FuchsKatharina Landfester
Oct 24, 2013·Nature Materials·Simona MuraPatrick Couvreur
Feb 25, 2014·International Journal of Biological Macromolecules·Daniel AgudeloHeidar-Ali Tajmir-Riahi
Feb 27, 2014·Journal of the American Chemical Society·Assaf J HarnoyRoey J Amir
Sep 25, 2014·Nanoscale·Quanyin HuZhen Gu
Dec 3, 2014·ACS Applied Materials & Interfaces·Arjyabaran SinhaNikhil R Jana
Jul 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Smita Kashyap, Manickam Jayakannan

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Citations

Oct 13, 2015·Biomaterials Science·Thavasyappan ThambiDoo Sung Lee
Aug 4, 2015·The Journal of Physical Chemistry. B·Poothayil Subash PramodManickam Jayakannan
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Yayuan GuanJianbin Luo
Oct 25, 2016·Chemical Society Reviews·Chunhua CaiLiquan Wang
Nov 16, 2019·Nature Communications·Jongha LeeDae-Hyeong Kim
Mar 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pinar Sinem Omurtag OzgenAydan Dag
Feb 5, 2021·Advanced Drug Delivery Reviews·Will Stuart-Walker, Clare S Mahon
Jul 24, 2021·Chemistry, an Asian Journal·Parvathy VenuRaja Shunmugam
Jan 10, 2017·Biomacromolecules·Nilesh Umakant Deshpande, Manickam Jayakannan
Nov 14, 2016·ACS Biomaterials Science & Engineering·Bapurao Surnar, Manickam Jayakannan
Dec 13, 2016·Biomacromolecules·Bapurao Surnar, Manickam Jayakannan
Jun 16, 2018·Biomacromolecules·Nilesh Umakant Deshpande, Manickam Jayakannan
Aug 2, 2018·ACS Applied Materials & Interfaces·Sangeun LeeAdah Almutairi

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