Dual target gene therapy to EML4-ALK NSCLC by a gold nanoshell-based system

Theranostics
Siwen LiYueqing Gu

Abstract

Although EML4-ALK transforming fusion gene is represented in only 8% of non-small cell lung cancer (NSCLC) cases, its expression is partly responsive for the failure of current NSCLC treatments. Preventing secondary mutation of the ALK protein through direct gene manipulation could overcome NSCLC drug resistance. Method: In this study, we developed a gold nanoshell (HAuNs) drug carrier for delivery and selective photo-thermal release of genes that target ALK and microRNA-301 in NSCLC. Additionally, the densely-coated nanoshell adsorbed high amounts of the positively-charged anticancer drug doxorubicin (DOX), generating an exciting multidimensional treatment strategy that includes gene-, thermal- and chemo- therapy. Results: The ALK mRNA and microRNA-301 genes as the double targets exhibited the combined effect. The drug carrier system significantly improved the drug accumulation in tumor tissues due to the enhanced vascular permeability by photothermal effect, dense spherical structure and RGD peptide modification. In vitro and in vivo results demonstrated the multiple therapeutic effects of the gold nanoshell-based system was better than the monotherapy. Conclusion: The above results indicated the gold nanoshell-based system w...Continue Reading

Citations

Oct 24, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Zhihang ChenZaver M Bhujwalla
Jul 24, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Wei WangYue-Yin Pan
Dec 4, 2020·Emerging Topics in Life Sciences·Ainoa GuinartTeresa D Tetley

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Methods Mentioned

BETA
transfection
electrophoresis
flow cytometry
transmission electron microscopy
confocal microscopy
fluorescence imaging

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