Dual-targeted contrast agent for US assessment of tumor angiogenesis in vivo.

Radiology
Jürgen K WillmannSanjiv S Gambhir

Abstract

To develop and validate a dual-targeted ultrasonographic (US) imaging agent with microbubbles (MBs) that attaches to both vascular endothelial growth factor (VEGF) receptor 2 (VEGFR2) and alpha(v)beta(3) integrin and to compare the US imaging signal obtained from dual-targeted MBs (MB(D)) with that from single-targeted MBs (MB(S)) in a murine model of tumor angiogenesis. Animal protocols were approved by the institutional Administrative Panel on Laboratory Animal Care. Single- and dual-targeted US imaging agents were prepared by attaching anti-VEGFR2, anti-alpha(v)beta(3) integrin, or both antibodies to the shell of perfluorocarbon-filled MBs. Binding specificities of targeted MBs compared with isotype-matched immunoglobulin G-labeled control MBs (MB(C)) and nontargeted nonlabeled MBs (MB(N)) were tested with VEGFR2-positive and alpha(v)beta(3) integrin-positive cells (mouse SVR cells) and control cells (mouse 4T1 cells). In vivo imaging signals of contrast material-enhanced US by using anti-VEGFR2-targeted MBs (MB(V)), anti-alpha(v)beta(3) integrin-targeted MBs (MB(I)), MB(D), and MB(C) were quantified in 49 mice bearing SK-OV-3 tumors (human ovarian cancer). Tumor tissue was stained for VEGFR2, alpha(v)beta(3) integrin, and C...Continue Reading

References

Nov 18, 1971·The New England Journal of Medicine·J Folkman
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Sep 27, 2001·Cell and Tissue Research·A van der Flier, A Sonnenberg
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Apr 2, 2003·Biophysical Journal·Sujata K BhatiaDaniel A Hammer
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Jul 2, 2003·Circulation·Dilantha B EllegalaJonathan R Lindner
Jun 4, 2004·The New England Journal of Medicine·Herbert HurwitzFairooz Kabbinavar
Dec 9, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel J Hicklin, Lee M Ellis
May 19, 2005·Cancer Research·Pia NybergRaghu Kalluri
Nov 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Douglas G McNeelGeorge Wilding
Dec 15, 2006·The New England Journal of Medicine·Alan SandlerDavid H Johnson
Jan 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Grzegorz KorpantyRolf A Brekken
Jan 12, 2007·The New England Journal of Medicine·Robert J MotzerRobert A Figlin
Jan 12, 2007·The New England Journal of Medicine·Bernard EscudierUNKNOWN TARGET Study Group

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Citations

Aug 4, 2010·Pediatric Radiology·Isabel KiesslingFabian Kiessling
May 13, 2010·European Journal of Nuclear Medicine and Molecular Imaging·J R Eisenbrey, F Forsberg
Apr 17, 2009·Journal of Neural Transmission·Brinda S DesaiBill Hendey
Jul 13, 2011·Journal of Neural Transmission·Brinda Desai BradaricBill Hendey
Jun 16, 2010·Angiogenesis·Nirupama DeshpandeJürgen K Willmann
Jun 1, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kentaro Otani, Kenichi Yamahara
Mar 3, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Malak Itani, Robert F Mattrey
Feb 21, 2009·Physics in Medicine and Biology·Shengping QinKatherine W Ferrara
Mar 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hua PanSamuel A Wickline
Aug 25, 2010·Investigative Radiology·Christopher R AndersonAlexander L Klibanov
Feb 24, 2011·Investigative Radiology·Christopher R AndersonJoshua J Rychak
Mar 21, 2013·Investigative Radiology·Jean-Marc HyvelinFrançois Tranquart
May 24, 2013·Investigative Radiology·Cedric M PanjeJürgen K Willmann
Aug 7, 2012·Interface Focus·F Stuart FosterS Lee Adamson
May 9, 2012·Journal of Biomedical Optics·Yu-Hsin WangPai-Chi Li
Jul 26, 2013·AJR. American Journal of Roentgenology·Andrei Iagaru, Sanjiv Sam Gambhir
Jul 25, 2012·AJR. American Journal of Roentgenology·Sunil Unnikrishnan, Alexander L Klibanov
Jul 28, 2012·International Journal of Molecular Sciences·Siver A MoestueRune Hansen
Jan 11, 2011·World Journal of Gastroenterology : WJG·Nischita K ReddyManoop S Bhutani
Oct 6, 2010·International Journal of Medical Sciences·Klaus BraunWaldemar Waldeck
May 7, 2011·Theranostics·Fabian KiesslingMoritz Palmowski
Nov 19, 2010·Radiology·Stephen B Solomon, Stuart G Silverman
Jul 24, 2012·Radiology·Moritz F Kircher, Jürgen K Willmann
May 25, 2012·Radiology·Moritz F Kircher, Jürgen K Willmann
Sep 16, 2014·Imaging in Medicine·Reshu Saini, Kenneth Hoyt
Dec 10, 2013·Advanced Drug Delivery Reviews·Fabian KiesslingTwan Lammers
Jun 17, 2010·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Gerrit L ten KateArend F L Schinkel
Jun 11, 2014·Cancer Metastasis Reviews·Jason M WarramEben L Rosenthal
Dec 14, 2011·Journal of Biomedicine & Biotechnology·A GrecoM Salvatore
Jul 16, 2010·Journal of Oncology·Grzegorz KorpantyRolf A Brekken
Apr 20, 2011·Radiology·Amelie M LutzSanjiv S Gambhir
Jun 26, 2009·Expert Opinion on Drug Delivery·Hui Wang, Xiaoyuan Chen
Jun 17, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Iason PapademetriouSilvia Muro

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