Dual-Targeting of miR-124-3p and ABCC4 Promotes Sensitivity to Adriamycin in Breast Cancer Cells.

Genetic Testing and Molecular Biomarkers
Di HuXinguang Qiu

Abstract

Increasing evidence links the abnormal expression of microRNAs and ATP-binding cassette subfamily C member 4 (ABCC4) with tumor development and progression, as well as with chemoresistance. Our aims were to determine the therapeutic potential of targeting both miR-124-3p and ABCC4 in breast cancer cells and to determine if duel targeting increased their sensitivity to chemotherapeutic drugs, in vitro. The expression of the ABCC4 protein and miR-124-3p were detected, respectively, by immunohistochemical staining and quantitative real-time polymerase chain reaction in breast cancer tumor tissue, MCF-7 and MCF-7-ADR cell lines. Suppression of ABCC4 expression and miR-124-3p overexpression were performed in MCF-7-ADR cell lines. Western blot assays were used to detect expression of ABCC4 and permeability glycoprotein 1/multi-drug resistance protein 1 (P-gp) in cells. Cell Counting Kit-8, flow cytometry, transwell, and scratch assays were conducted to detect cell proliferation, cell cycle, invasion, and migration of cells. We found that ABCC4 protein expression was significantly increased, while the miR-124-3p level was significantly decreased in breast cancer tissue and cell lines. Tumor size and clinical tumor node metastasis stag...Continue Reading

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Citations

Aug 8, 2019·Cancer Medicine·Xinqi JiaShan Yu
Mar 4, 2020·Molecular Cancer·Xinyi ZhangYongguang Tao
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Apr 17, 2021·Cancer Cell International·Amir Sadra ZangoueiMeysam Moghbeli
Apr 21, 2021·Molecular Biology Reports·Qian LiFrederick T Greenaway
May 5, 2021·The Journal of Pharmacy and Pharmacology·Khadijeh JamialahmadiGholamreza Karimi
Sep 16, 2021·Cancer Metastasis Reviews·Iris Garrido-CanoPilar Eroles
Oct 1, 2021·Frontiers in Oncology·Jin-Hai TianLi-Bin Wang
Sep 24, 2021·Methods : a Companion to Methods in Enzymology·Xin WangMaozu Guo

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Methods Mentioned

BETA
xenografts
PCR
Assay
transfection

Software Mentioned

ImageJ
SPSS

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