Dual two-component regulatory systems are involved in aromatic compound degradation in a polychlorinated-biphenyl degrader, Rhodococcus jostii RHA1.

Journal of Bacteriology
Hisashi TakedaMasao Fukuda

Abstract

A Gram-positive polychlorinated-biphenyl (PCB) degrader, Rhodococcus jostii RHA1, degrades PCBs by cometabolism with biphenyl. A two-component BphS1T1 system encoded by bphS1 and bphT1 (formerly bphS and bphT) is responsible for the transcription induction of the five gene clusters, bphAaAbAcAdC1B1, etbAa1Ab1CbphD1, etbAa2Ab2AcD2, etbAdbphB2, and etbD1, which constitute multiple enzyme systems for biphenyl/PCB degradation. The bphS2 and bphT2 genes, which encode BphS2 and BphT2, virtually identical to BphS1 (92%) and BphT1 (97%), respectively, were characterized. BphS2T2 induced the activation of the bphAa promoter in a host, Rhodococcus erythropolis IAM1399, in the presence of a variety of aromatics, including benzene, toluene, ethylbenzene, xylenes, isopropylbenzene, and chlorinated benzenes, as effectively as BphS1T1. The substrate spectrum of BphS2T2 was the same as that of BphS1T1, except for biphenyl, which is a substrate only for BphS1T1. BphS2T2 activated transcription from the five promoters of biphenyl/PCB degradation enzyme gene clusters as effectively as BphS1T1. The targeted disruptions of the bphS1, bphS2, bphT1, and bphT2 genes indicated that all these genes are involved in the growth of RHA1 on aromatic compound...Continue Reading

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Citations

Feb 4, 2012·The Journal of Biological Chemistry·J Andrés ValderramaEduardo Díaz
Feb 2, 2016·Bioscience, Biotechnology, and Biochemistry·Yuki AtagoHirofumi Hara
Feb 19, 2013·Journal of Bioscience and Bioengineering·Phuong Anh Thi NguyenMasao Fukuda
Dec 3, 2011·Journal of Bioscience and Bioengineering·Jun ShimodairaMasao Fukuda
May 23, 2015·Genome Announcements·Elham EhsaniRamiro Vilchez-Vargas
Nov 22, 2014·Bioscience, Biotechnology, and Biochemistry·Kenichi YanoNoritaka Iwai
Feb 6, 2020·FEMS Microbiology Reviews·Bruno MaucourtFrançoise Bringel
Jan 31, 2021·Biotechnology Advances·Miroslav PátekJan Nešvera

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