Dual use of immunohistochemistry for film densitometry and light microscopy.

Journal of Neuroscience Methods
Miguel Melendez-FerroEmma Perez-Costas

Abstract

In the present study, we applied the principles of immunoblotting and light microscopy immunohistochemistry to develop a combined methodology that allows obtaining optical density data in films, as well as morphological and protein distribution data on slides using the same brain tissue section, thus maximizing the data obtained from a single sample. This is especially important when experiments are performed using very valuable or unique tissue samples, which is a very common case in the study of the human brain. The ideal methodology should combine the possibility of measuring levels of expression of a marker, and the capability to map accurately the distribution of that marker in the region of interest. To achieve this, two things are required: first, the technique needs to be sensitive enough to obtain optical density or intensity measurements of the marker, and second, a good preservation of the tissue is needed for the study of distribution patterns and morphological analysis. Here we show that our combined methodology produced reliable results for different tissue preservation conditions (fresh-frozen and fixed tissue), in different species (rat and human), in different brain areas (substantia nigra and striatum), and fo...Continue Reading

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Citations

Apr 23, 2013·Animal Reproduction Science·F MoreiraT Lucia
Jan 8, 2016·Brain Structure & Function·Lesley A McCollumRosalinda C Roberts
Dec 30, 2014·Journal of Ovarian Research·Augusto SchneiderMichal M Masternak
Dec 14, 2018·Ecotoxicology and Environmental Safety·Yang EWenfu Chen

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